Source:http://linkedlifedata.com/resource/pubmed/id/12851398
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
38
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| pubmed:dateCreated |
2003-9-15
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| pubmed:abstractText |
Glycosylphosphatidylinositol (GPI)-anchored proteins are ubiquitous in eukaryotes. The minimum conserved GPI core structure of all GPI-anchored glycans has been determined as EtN-PO4-6Manalpha1-2Manalpha1-6Manalpha1-4GlcN-myo-inositol-PO3H. Human placental alkaline phosphatase (AP) has been reported to be a GPI-anchored membrane protein. AP carries one N-glycan, (NeuAcalpha2-->3)2Gal2GlcNAc2Man3GlcNAc(+/-Fuc)GlcNAc, and a GPI anchor, which contains an ethanolamine phosphate diester group, as a side chain. However, we found that both sialidase-treated soluble AP (sAP) and its GPI-anchored glycan bound to a Psathyrella velutina lectin (PVL)-Sepharose column, which binds beta-GlcNAc residues. PVL binding of asialo-sAP and its GPI-anchored glycan was diminished by digestion with diplococcal beta-N-acetylhexosaminidase or by mild acid treatment. After sequential digestion of asialo-sAP with beta-N-acetylhexosaminidase and acid phosphatase, the elution patterns on chromatofocusing gels were changed in accordance with the negative charges of phosphate residues. Trypsin-digested sAP was analyzed by liquid chromatography/electrospray ionization mass spectrometry, and the structures of two glycopeptides with GPI-anchored glycans were confirmed as peptide-EtN-PO4-6Manalpha1-->2(GlcNAcbeta1-PO4-->6)Manalpha1-6(+/-EtN-PO4-->)Manalpha1-->4GlcN, which may be produced by endo-alpha-glucosaminidase. In addition to AP, GPI-anchored carcinoembryonic antigen, cholinesterase, and Tamm-Horsfall glycoprotein also bound to a PVL-Sepharose column, suggesting that the beta-N-acetylglucosaminyl phosphate diester residue is widely distributed in human GPI-anchored glycans. Furthermore, we found that the beta-N-acetylglucosaminyl phosphate diester residue is important for GPI anchor recognition of aerolysin, a channel-forming toxin derived from Aeromonas hydrophila.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Alkaline Phosphatase,
http://linkedlifedata.com/resource/pubmed/chemical/Bacterial Toxins,
http://linkedlifedata.com/resource/pubmed/chemical/Esters,
http://linkedlifedata.com/resource/pubmed/chemical/Ethanolamines,
http://linkedlifedata.com/resource/pubmed/chemical/Glycosylphosphatidylinositols,
http://linkedlifedata.com/resource/pubmed/chemical/Hexosaminidases,
http://linkedlifedata.com/resource/pubmed/chemical/Monosaccharides,
http://linkedlifedata.com/resource/pubmed/chemical/Neuraminidase,
http://linkedlifedata.com/resource/pubmed/chemical/Oxygen,
http://linkedlifedata.com/resource/pubmed/chemical/Peptides,
http://linkedlifedata.com/resource/pubmed/chemical/Pore Forming Cytotoxic Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Sepharose,
http://linkedlifedata.com/resource/pubmed/chemical/Trypsin,
http://linkedlifedata.com/resource/pubmed/chemical/aerolysin
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| pubmed:status |
MEDLINE
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| pubmed:month |
Sep
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| pubmed:issn |
0021-9258
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:day |
19
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| pubmed:volume |
278
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
36296-303
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| pubmed:dateRevised |
2006-11-15
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| pubmed:meshHeading |
pubmed-meshheading:12851398-Aeromonas,
pubmed-meshheading:12851398-Alkaline Phosphatase,
pubmed-meshheading:12851398-Amino Acid Sequence,
pubmed-meshheading:12851398-Bacterial Toxins,
pubmed-meshheading:12851398-Carbohydrate Sequence,
pubmed-meshheading:12851398-Dose-Response Relationship, Drug,
pubmed-meshheading:12851398-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:12851398-Esters,
pubmed-meshheading:12851398-Ethanolamines,
pubmed-meshheading:12851398-Glycosylphosphatidylinositols,
pubmed-meshheading:12851398-Hexosaminidases,
pubmed-meshheading:12851398-Humans,
pubmed-meshheading:12851398-Mass Spectrometry,
pubmed-meshheading:12851398-Molecular Sequence Data,
pubmed-meshheading:12851398-Monosaccharides,
pubmed-meshheading:12851398-Neuraminidase,
pubmed-meshheading:12851398-Oxygen,
pubmed-meshheading:12851398-Peptides,
pubmed-meshheading:12851398-Placenta,
pubmed-meshheading:12851398-Pore Forming Cytotoxic Proteins,
pubmed-meshheading:12851398-Protein Binding,
pubmed-meshheading:12851398-Sepharose,
pubmed-meshheading:12851398-Spectrometry, Mass, Electrospray Ionization,
pubmed-meshheading:12851398-Surface Plasmon Resonance,
pubmed-meshheading:12851398-Time Factors,
pubmed-meshheading:12851398-Trypsin
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| pubmed:year |
2003
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| pubmed:articleTitle |
A beta-N-acetylglucosaminyl phosphate diester residue is attached to the glycosylphosphatidylinositol anchor of human placental alkaline phosphatase: a target of the channel-forming toxin aerolysin.
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| pubmed:affiliation |
Department of Biochemistry, Sasaki Institute, 2-2 Kanda-Surugadai, Chiyoda-ku, Tokyo 101-0062, Japan.
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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