Source:http://linkedlifedata.com/resource/pubmed/id/12846554
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
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| pubmed:dateCreated |
2003-7-8
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| pubmed:abstractText |
The synovial membrane (SM) is a source of multipotent mesenchymal stem cells (MSCs), which appeared microscopically to be a relatively homogeneous population of fibroblast-like cells (FCs) in culture (De Bari et al., 2001). The aim of this study was to investigate phenotypic characteristics of the SM-derived FCs (SD-FCs) that could elucidate their origin inside the synovial tissue. Morphological characterization of SD-FCs was assessed by electron microscopy and by expression of surfactant protein A (SPA). This study, yielded substantial evidence that SD-FCs show ultrastructural and immunocytochemical features of type B synoviocytes; they contained characteristic lamellar bodies (LBs) that are secreted by exocytosis. LB secretion ability was maintained upon passaging (P3-P10). Immunocytochemistry showed that SD-FCs express surfactant protein A (SP-A). Taken together, these results indicate that multipotent SD-MSCs may originate from the synovial lining, having a phenotype highly similar to that of type B synoviocytes. We believe our data highlight the potent ability of type B synoviocytes to have a multilineage differentiation potential.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical | |
| pubmed:status |
MEDLINE
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| pubmed:month |
May
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| pubmed:issn |
0914-9465
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:volume |
66
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
145-53
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| pubmed:dateRevised |
2004-11-17
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| pubmed:meshHeading |
pubmed-meshheading:12846554-Adipocytes,
pubmed-meshheading:12846554-Cell Differentiation,
pubmed-meshheading:12846554-Cell Lineage,
pubmed-meshheading:12846554-Cells, Cultured,
pubmed-meshheading:12846554-Chondrocytes,
pubmed-meshheading:12846554-Exocytosis,
pubmed-meshheading:12846554-Fibroblasts,
pubmed-meshheading:12846554-Humans,
pubmed-meshheading:12846554-Immunohistochemistry,
pubmed-meshheading:12846554-Mesoderm,
pubmed-meshheading:12846554-Oligodendroglia,
pubmed-meshheading:12846554-Pseudopodia,
pubmed-meshheading:12846554-Pulmonary Surfactant-Associated Protein A,
pubmed-meshheading:12846554-Stem Cells,
pubmed-meshheading:12846554-Synovial Membrane
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| pubmed:year |
2003
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| pubmed:articleTitle |
Morphological and immunocytochemical characterization of cultured fibroblast-like cells derived from adult human synovial membrane.
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| pubmed:affiliation |
Laboratory of Histology, Biomedical Research Institute-DWI, Limburgs Universitaire Centrum, Diepenbeek, Belgium. frank.vandenabeele@luc.ac.be
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| pubmed:publicationType |
Journal Article
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