Source:http://linkedlifedata.com/resource/pubmed/id/12844500
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
4
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| pubmed:dateCreated |
2003-9-12
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| pubmed:abstractText |
The objective of this study was to assess the potential of adeno-associated virus (AAV)-mediated gene delivery into coronary microvessels in vivo in a large animal. Ten mongrel dogs were chronically instrumented and allowed to recover for 10 days. Dogs were reanesthetized, and the aorta was constricted by a hydraulic occluder, whereby left ventricular (LV) pressure increased by 30% and left circumflex coronary artery blood flow by 50%. Recombinant AAV (serotype 2, CMV enhancer/chicken beta-actin promoter) encoding for green fluorescent protein (GFP) was injected as a bolus into the left atrium during aortic constriction at total titers of 1010 or 1012 infectious units. Dogs were followed for 2 (n = 4)or4wk(n = 6). Hemodynamics or body weight did not change. In LV tissue slices, a fluorescein-labeled antibody to GFP stained endothelial and smooth muscle cells but was absent in myocytes. To quantify transduction, slices were then stained with antibodies against alpha-smooth muscle actin or von Willebrand factor. Approximately 4% of arterioles and 2% of microvessels stained positive for anti-GFP independent from viral titer or duration. By regression analyses, the percent of vessels transfected was proportional to the increase in LV systolic pressure during occlusion. AAV is a potential vector for gene transfer into the coronary microcirculation in large animals, including perhaps humans.
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| pubmed:grant |
http://linkedlifedata.com/resource/pubmed/grant/AG-17042,
http://linkedlifedata.com/resource/pubmed/grant/HL-39902,
http://linkedlifedata.com/resource/pubmed/grant/HL-61290,
http://linkedlifedata.com/resource/pubmed/grant/HL-62573,
http://linkedlifedata.com/resource/pubmed/grant/HL-65577,
http://linkedlifedata.com/resource/pubmed/grant/P0-1 HL-59412,
http://linkedlifedata.com/resource/pubmed/grant/P01 HL-43023,
http://linkedlifedata.com/resource/pubmed/grant/R01 HL-50142
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical | |
| pubmed:status |
MEDLINE
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| pubmed:month |
Oct
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| pubmed:issn |
8750-7587
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:volume |
95
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
1688-94
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| pubmed:dateRevised |
2007-11-15
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| pubmed:meshHeading |
pubmed-meshheading:12844500-Animals,
pubmed-meshheading:12844500-Coronary Vessels,
pubmed-meshheading:12844500-Dependovirus,
pubmed-meshheading:12844500-Dogs,
pubmed-meshheading:12844500-Endothelium, Vascular,
pubmed-meshheading:12844500-Gene Transfer Techniques,
pubmed-meshheading:12844500-Genetic Vectors,
pubmed-meshheading:12844500-Green Fluorescent Proteins,
pubmed-meshheading:12844500-Hemodynamics,
pubmed-meshheading:12844500-Immunohistochemistry,
pubmed-meshheading:12844500-Indicators and Reagents,
pubmed-meshheading:12844500-Leukocyte Count,
pubmed-meshheading:12844500-Luminescent Proteins,
pubmed-meshheading:12844500-Microscopy, Confocal,
pubmed-meshheading:12844500-Myocytes, Smooth Muscle,
pubmed-meshheading:12844500-Neutrophils,
pubmed-meshheading:12844500-Tissue Distribution,
pubmed-meshheading:12844500-Transduction, Genetic
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| pubmed:year |
2003
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| pubmed:articleTitle |
Adeno-associated virus mediated gene delivery into coronary microvessels of chronically instrumented dogs.
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| pubmed:affiliation |
Dept. of Physiology, Basic Sciences Bldg., Rm. 636, New York Medical College, Valhalla, NY 10595, USA.
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| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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