Linked Life Data

12839093

Source:http://linkedlifedata.com/resource/pubmed/id/12839093

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2003-7-3
pubmed:abstractText
Although nature's design of the DNA double helix is ingenious for the storage of information, this design creates topological problems for the processes that occur on the DNA. Cellular processes, such as transcription, replication, chromosomal segregation, and chromosomal condensation, are all complicated by the double helix. The problem is compounded in cells since the DNA is in chromatin. Topoisomerases relax positive and negative superhelical turns in DNA, and thereby topoisomerases have long been recognized as key components of the DNA replication and chromosome segregation and condensation machinery. A role for topoisomerases in the transcription process has also been noted in living cells, but only recently has such a role been recapitulated in the test tube for transcription reactions. New data are discussed that demonstrate that for in vitro transcription reactions, topoisomerases are dispensable when the template is naked DNA, but when the template is reconstituted into chromatin, topoisomerases are required for transcription to proceed efficiently.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
1045-4403
pubmed:author
pubmed:issnType
Print
pubmed:volume
13
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1-8
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
2003
pubmed:articleTitle
Transcription from the perspective of the DNA: twists and bumps in the road.
pubmed:affiliation
Department of Pathology, Brigham and Women's Hospital and Harvard Medical School, MA 02115, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Review