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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1-2
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pubmed:dateCreated |
2003-7-1
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pubmed:abstractText |
Glutamate exposure of astroglial cells caused ligand-gated channel receptor activation, associated with excitotoxic cell response. We investigated the effects of 24 h glutamate exposure on transglutaminase in astrocytes primary cultures at 7, 14, and 21 days in vitro (DIV). Increases in enzyme activity were observed as a function of cell differentiation stage in glutamate-treated cultures. These effects were significantly reduced when GYKI 52466, an AMPA/KA receptors inhibitor, was added to the culture medium prior to incubation with glutamate. Microscopy observation on transglutaminase-mediated, fluorescent dansylcadaverine incorporation in living cells was consistent with these results. Western blotting analysis with monoclonal antibody showed that glutamate also up-regulated tissue transglutaminase expression, which reached the highest values in 14 DIV cultures. Confocal laser scanning microscopy analysis of immunostained astroglial cells showed a mainly cytoplasmic localisation of the enzyme both in control and treated cultures; nevertheless, counterstaining with the nuclear dye acridine orange demonstrated the presence of tissue transglutaminase also into the nucleus of glutamate-exposed and 21 DIV cells. The increases in enzyme expression and localisation in the nucleus of glutamate-treated astroglial cells may be part of biochemical alterations induced by excitotoxic stimulus.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0006-8993
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
18
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pubmed:volume |
978
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
24-30
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:12834894-Animals,
pubmed-meshheading:12834894-Animals, Newborn,
pubmed-meshheading:12834894-Anti-Anxiety Agents,
pubmed-meshheading:12834894-Astrocytes,
pubmed-meshheading:12834894-Benzodiazepines,
pubmed-meshheading:12834894-Blotting, Western,
pubmed-meshheading:12834894-Cadaverine,
pubmed-meshheading:12834894-Cells, Cultured,
pubmed-meshheading:12834894-Dose-Response Relationship, Drug,
pubmed-meshheading:12834894-Drug Interactions,
pubmed-meshheading:12834894-Excitatory Amino Acid Agents,
pubmed-meshheading:12834894-Excitatory Amino Acid Antagonists,
pubmed-meshheading:12834894-Glutamate-Ammonia Ligase,
pubmed-meshheading:12834894-Glutamic Acid,
pubmed-meshheading:12834894-Immunohistochemistry,
pubmed-meshheading:12834894-Microscopy, Confocal,
pubmed-meshheading:12834894-Rats,
pubmed-meshheading:12834894-Rats, Wistar,
pubmed-meshheading:12834894-Time Factors,
pubmed-meshheading:12834894-Transglutaminases
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pubmed:year |
2003
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pubmed:articleTitle |
Glutamate-induced increases in transglutaminase activity in primary cultures of astroglial cells.
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pubmed:affiliation |
Department of Biological Chemistry, Medical Chemistry and Molecular Biology, University of Catania, Catania, Italy.
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pubmed:publicationType |
Journal Article,
Comparative Study
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