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rdf:type | |
lifeskim:mentions | |
pubmed:dateCreated |
1993-2-12
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pubmed:abstractText |
The aim of the present experiments was to test the possible involvement of nitric oxide (NO) in cytokine-induced enhancement of tumor cell (TC) adhesion to endothelial cells (ECs). Exposure of EA hyb 926 cells to TNF (500 U/ml) plus IFN (100 U/ml) for 24 h significantly enhanced their adhesivity for the 51Cr-labeled GLC1 (small cell lung carcinoma) TCs. Conversely, exposure of TCs to cytokines did not result in an increased adhesion of these cells to ECs. TC-stimulated adhesion to EA hyb 926 was abrogated by the glucocorticoid dexamethasone (Dex, 10(-7) M), the NO synthase inhibitors N omega-nitro-L-arginine methyl ester (L-NAME, 10(-5) M) and NG-monomethyl-L-arginine (L-NMMA, 10(-5) M) and the protein synthesis inhibitor cycloheximide (Cex, 10(-6) M). Furthermore, GLC1-stimulated adhesion to EA hyb 926 was reversed following removal of L-arginine from the medium or pretreatment with the guanylate cyclase inhibitor methylene blue. TC-stimulated adhesion was also prevented when TCs were pretreated with the monoclonal antibody CD15 directed against the endothelial-leukocyte adhesion molecule (ELAM-1) ligand or following exposure of ECs to anti-ELAM-1 monoclonal antibody. Although suppressing TC-stimulated adhesion, L-NMMA failed to modify significantly cytokine-induced ELAM-1 expression in EA hyb 926. These results (a) provide evidence for the NO-inducible pathway contributing to cytokine-induced enhancement of tumor cell adhesion to the vascular endothelium and (b) demonstrate the involvement of the ELAM-1/CD15 adhesion system in tumor cell-stimulated adhesion to ECs.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Arginine,
http://linkedlifedata.com/resource/pubmed/chemical/Cell Adhesion Molecules,
http://linkedlifedata.com/resource/pubmed/chemical/Cycloheximide,
http://linkedlifedata.com/resource/pubmed/chemical/Cytokines,
http://linkedlifedata.com/resource/pubmed/chemical/Dexamethasone,
http://linkedlifedata.com/resource/pubmed/chemical/E-Selectin,
http://linkedlifedata.com/resource/pubmed/chemical/Methylene Blue,
http://linkedlifedata.com/resource/pubmed/chemical/NG-Nitroarginine Methyl Ester,
http://linkedlifedata.com/resource/pubmed/chemical/Nitric Oxide,
http://linkedlifedata.com/resource/pubmed/chemical/omega-N-Methylarginine
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pubmed:status |
MEDLINE
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pubmed:issn |
0160-2446
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
20 Suppl 12
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
S155-9
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:1282956-Arginine,
pubmed-meshheading:1282956-Cell Adhesion,
pubmed-meshheading:1282956-Cell Adhesion Molecules,
pubmed-meshheading:1282956-Cell Line,
pubmed-meshheading:1282956-Cycloheximide,
pubmed-meshheading:1282956-Cytokines,
pubmed-meshheading:1282956-Dexamethasone,
pubmed-meshheading:1282956-E-Selectin,
pubmed-meshheading:1282956-Endothelium, Vascular,
pubmed-meshheading:1282956-Humans,
pubmed-meshheading:1282956-Methylene Blue,
pubmed-meshheading:1282956-NG-Nitroarginine Methyl Ester,
pubmed-meshheading:1282956-Nitric Oxide,
pubmed-meshheading:1282956-Tumor Cells, Cultured,
pubmed-meshheading:1282956-omega-N-Methylarginine
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pubmed:year |
1992
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pubmed:articleTitle |
Involvement of nitric oxide in tumor cell adhesion to cytokine-activated endothelial cells.
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pubmed:affiliation |
Department of Cardiology, Instituto Scientifico San Raffaele, Milan, Italy.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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