Source:http://linkedlifedata.com/resource/pubmed/id/12827359
Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
6
|
| pubmed:dateCreated |
2003-9-23
|
| pubmed:abstractText |
Activation of KCNQ potassium channels by stimulation of co-expressed dopamine D(2) receptors was studied electrophysiologically in Xenopus laevis oocytes and in mammalian cells. To address the specificity of the interaction between D(2)-like receptors and KCNQ channels, combinations of KCNQ1-5 channels and D(2)-like receptors (D(2L), D(3), and D(4)) were investigated in Xenopus oocytes. Activation of either receptor with the selective D(2)-like receptor agonist quinpirole (100 nM) stimulated all the KCNQ currents, independently of the subunit combination, indicating a common pathway of receptor-channel interaction. The KCNQ4 current was investigated in further detail and was increased by 19.9+/-1.6% ( n=20) by D(2L) receptor stimulation. The effect could be mimicked by injection of GTPgammaS and prevented by injection of Bordetella pertussis toxin, indicating that channel stimulation was mediated via a G protein of the G(alphai/o) subtype. Cells of the human neuroblastoma line SH-SY5Y were co-transfected transiently with KCNQ4 and D(2L) receptors. Stimulation of D(2L) receptors increased the KCNQ4 current ( n=6) as determined in whole-cell patch-clamp recordings. The specificity of the dopaminergic activation of the KCNQ channels was confirmed by co-expression of other neuronal K(+) channels (BK, K(V)1.1, and K(V)4.3) with the D(2L) receptor in Xenopus oocytes. None of these K(+) channels responded to stimulation of the D(2L) receptor. In the mammalian brain, dopamine D(2) receptors and KCNQ channels co-localise postsynaptically in several brain regions, so modulation of neuronal excitability by dopamine release could in part be mediated via an effect on KCNQ channels.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Complementary,
http://linkedlifedata.com/resource/pubmed/chemical/Dopamine Agonists,
http://linkedlifedata.com/resource/pubmed/chemical/Guanosine 5'-O-(3-Thiotriphosphate),
http://linkedlifedata.com/resource/pubmed/chemical/Pertussis Toxin,
http://linkedlifedata.com/resource/pubmed/chemical/Potassium Channels,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Dopamine D2
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Sep
|
| pubmed:issn |
0031-6768
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
446
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
684-94
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:12827359-Animals,
pubmed-meshheading:12827359-Biotransformation,
pubmed-meshheading:12827359-Brain Neoplasms,
pubmed-meshheading:12827359-Cell Line, Tumor,
pubmed-meshheading:12827359-DNA, Complementary,
pubmed-meshheading:12827359-Dopamine Agonists,
pubmed-meshheading:12827359-Electrophysiology,
pubmed-meshheading:12827359-Guanosine 5'-O-(3-Thiotriphosphate),
pubmed-meshheading:12827359-Humans,
pubmed-meshheading:12827359-Membrane Potentials,
pubmed-meshheading:12827359-Neuroblastoma,
pubmed-meshheading:12827359-Neurons,
pubmed-meshheading:12827359-Oocytes,
pubmed-meshheading:12827359-Patch-Clamp Techniques,
pubmed-meshheading:12827359-Pertussis Toxin,
pubmed-meshheading:12827359-Potassium Channels,
pubmed-meshheading:12827359-Receptors, Dopamine D2,
pubmed-meshheading:12827359-Transfection,
pubmed-meshheading:12827359-Xenopus laevis
|
| pubmed:year |
2003
|
| pubmed:articleTitle |
Functional coupling between heterologously expressed dopamine D(2) receptors and KCNQ channels.
|
| pubmed:affiliation |
Department of Medical Physiology, The Panum Institute, University of Copenhagen, Blegdamsvej 3C, Building 12.5, 2200, Copenhagen N, Denmark.
|
| pubmed:publicationType |
Journal Article,
In Vitro,
Research Support, Non-U.S. Gov't
|