Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
13
pubmed:dateCreated
2003-6-25
pubmed:abstractText
DNA microarrays are widely used in biological research; by analyzing differential hybridization on a single microarray slide, one can detect changes in mRNA expression levels, increases in DNA copy numbers and the location of transcription factor binding sites on a genomic scale. Having performed the experiments, the major challenge is to process large, noisy datasets in order to identify the specific array elements that are significantly differentially hybridized. This normally requires aggregating different, often incompatible programs into a multi-step pipeline. Here we present ExpressYourself, a fully integrated platform for processing microarray data. In completely automated fashion, it will correct the background array signal, normalize the Cy5 and Cy3 signals, score levels of differential hybridization, combine the results of replicate experiments, filter problematic regions of the array and assess the quality of individual and replicate experiments. ExpressYourself is designed with a highly modular architecture so various types of microarray analysis algorithms can readily be incorporated as they are developed; for example, the system currently implements several normalization methods, including those that simultaneously consider signal intensity and slide location. The processed data are presented using a web-based graphical interface to facilitate comparison with the original images of the array slides. In particular, Express Yourself is able to regenerate images of the original microarray after applying various steps of processing, which greatly facilities identification of position-specific artifacts. The program is freely available for use at http://bioinfo.mbb.yale.edu/expressyourself.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/12824348-10077610, http://linkedlifedata.com/resource/pubmed/commentcorrection/12824348-10359783, http://linkedlifedata.com/resource/pubmed/commentcorrection/12824348-10371154, http://linkedlifedata.com/resource/pubmed/commentcorrection/12824348-10391217, http://linkedlifedata.com/resource/pubmed/commentcorrection/12824348-10471496, http://linkedlifedata.com/resource/pubmed/commentcorrection/12824348-10521349, http://linkedlifedata.com/resource/pubmed/commentcorrection/12824348-10684945, http://linkedlifedata.com/resource/pubmed/commentcorrection/12824348-10969801, http://linkedlifedata.com/resource/pubmed/commentcorrection/12824348-11042457, http://linkedlifedata.com/resource/pubmed/commentcorrection/12824348-11050323, http://linkedlifedata.com/resource/pubmed/commentcorrection/12824348-11125145, http://linkedlifedata.com/resource/pubmed/commentcorrection/12824348-11197775, http://linkedlifedata.com/resource/pubmed/commentcorrection/12824348-11206552, http://linkedlifedata.com/resource/pubmed/commentcorrection/12824348-11309499, http://linkedlifedata.com/resource/pubmed/commentcorrection/12824348-11410663, http://linkedlifedata.com/resource/pubmed/commentcorrection/12824348-11470890, http://linkedlifedata.com/resource/pubmed/commentcorrection/12824348-11473005, http://linkedlifedata.com/resource/pubmed/commentcorrection/12824348-11544189, http://linkedlifedata.com/resource/pubmed/commentcorrection/12824348-11571077, http://linkedlifedata.com/resource/pubmed/commentcorrection/12824348-11779829, http://linkedlifedata.com/resource/pubmed/commentcorrection/12824348-11808746, http://linkedlifedata.com/resource/pubmed/commentcorrection/12824348-11827952, http://linkedlifedata.com/resource/pubmed/commentcorrection/12824348-11842121, http://linkedlifedata.com/resource/pubmed/commentcorrection/12824348-11931570, http://linkedlifedata.com/resource/pubmed/commentcorrection/12824348-12016056, http://linkedlifedata.com/resource/pubmed/commentcorrection/12824348-12073330, http://linkedlifedata.com/resource/pubmed/commentcorrection/12824348-12074162, http://linkedlifedata.com/resource/pubmed/commentcorrection/12824348-12136105, http://linkedlifedata.com/resource/pubmed/commentcorrection/12824348-12184807, http://linkedlifedata.com/resource/pubmed/commentcorrection/12824348-12184811, http://linkedlifedata.com/resource/pubmed/commentcorrection/12824348-12192590, http://linkedlifedata.com/resource/pubmed/commentcorrection/12824348-12216114, http://linkedlifedata.com/resource/pubmed/commentcorrection/12824348-12225587, http://linkedlifedata.com/resource/pubmed/commentcorrection/12824348-12399584, http://linkedlifedata.com/resource/pubmed/commentcorrection/12824348-12429061, http://linkedlifedata.com/resource/pubmed/commentcorrection/12824348-12454644, http://linkedlifedata.com/resource/pubmed/commentcorrection/12824348-12454645, http://linkedlifedata.com/resource/pubmed/commentcorrection/12824348-12454651, http://linkedlifedata.com/resource/pubmed/commentcorrection/12824348-7569999, http://linkedlifedata.com/resource/pubmed/commentcorrection/12824348-8855227, http://linkedlifedata.com/resource/pubmed/commentcorrection/12824348-8944026, http://linkedlifedata.com/resource/pubmed/commentcorrection/12824348-9351342, http://linkedlifedata.com/resource/pubmed/commentcorrection/12824348-9381177, http://linkedlifedata.com/resource/pubmed/commentcorrection/12824348-9415887, http://linkedlifedata.com/resource/pubmed/commentcorrection/12824348-9784122, http://linkedlifedata.com/resource/pubmed/commentcorrection/12824348-9843981, http://linkedlifedata.com/resource/pubmed/commentcorrection/12824348-9889134, http://linkedlifedata.com/resource/pubmed/commentcorrection/12824348-9915498
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
1362-4962
pubmed:author
pubmed:issnType
Electronic
pubmed:day
1
pubmed:volume
31
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
3477-82
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
2003
pubmed:articleTitle
ExpressYourself: A modular platform for processing and visualizing microarray data.
pubmed:affiliation
Department of Molecular Biophysics and Biochemistry, Yale University, 266 Whitney Avenue, PO Box 208114, New Haven CT 06520-8114, USA. nicholas.luscombe@yale.edu
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't