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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
6
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pubmed:dateCreated |
2003-6-24
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pubmed:abstractText |
We show that the Escherichia coli RNA polymerase (RNAP) alpha subunit C-terminal domain (alphaCTD) functionally interacts with sigma(70) at a subset of UP-element- and activator-dependent promoters, we define the determinants of alphaCTD and sigma(70) required for the interaction, and we present a structural model for the interaction. The alphaCTD-sigma(70) interaction spans the upstream promoter and core promoter, thereby linking recognition of UP-elements and activators in the upstream promoter with recognition of the -35 element in the core promoter. We propose that the alphaCTD-sigma(70) interaction permits UP-elements and activators not only to "recruit" RNAP through direct interaction with alphaCTD, but also to "remodel" RNAP-core-promoter interaction through indirect, alphaCTD-bridged interactions with sigma(70).
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
1097-2765
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
11
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1621-33
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:12820974-Alanine,
pubmed-meshheading:12820974-Amino Acid Sequence,
pubmed-meshheading:12820974-Amino Acid Substitution,
pubmed-meshheading:12820974-Bacterial Proteins,
pubmed-meshheading:12820974-Binding Sites,
pubmed-meshheading:12820974-DNA, Bacterial,
pubmed-meshheading:12820974-DNA-Directed RNA Polymerases,
pubmed-meshheading:12820974-Escherichia coli,
pubmed-meshheading:12820974-Gene Expression Regulation, Bacterial,
pubmed-meshheading:12820974-Genes, Bacterial,
pubmed-meshheading:12820974-Genes, Suppressor,
pubmed-meshheading:12820974-Models, Molecular,
pubmed-meshheading:12820974-Molecular Sequence Data,
pubmed-meshheading:12820974-Mutagenesis,
pubmed-meshheading:12820974-Protein Structure, Tertiary,
pubmed-meshheading:12820974-Sigma Factor,
pubmed-meshheading:12820974-Transcription, Genetic,
pubmed-meshheading:12820974-Transcriptional Activation
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pubmed:year |
2003
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pubmed:articleTitle |
Functional interaction between RNA polymerase alpha subunit C-terminal domain and sigma70 in UP-element- and activator-dependent transcription.
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pubmed:affiliation |
Waksman Institute, Department of Chemistry, Rutgers University, Piscataway, NJ 08854, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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