Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
7
pubmed:dateCreated
2003-6-23
pubmed:abstractText
Outer membrane proteins (OMPs) are incorporated into the outer plasma membrane of Helicobacter pylori and are important for, e.g., ion transport, adherence, structural and osmotic stability, and bacterial virulence but may also be antigenic due to their surface exposure. Previous proteome-based approaches with H. pylori lysates determined a strong serological reaction towards two H. pylori OMPs, HpaA (TIGR HP0797) and Omp18 (TIGR HP1125). PCR was used to detect DNA encoding the two proteins, and a positive signal was found in all H. pylori strains tested. Proteins were cloned and expressed in the human kidney cell line HK293 with the QiaExpressionist system with a C-terminal His tag. Only sera from infected persons showed a positive reaction with the recombinant proteins. Recombinant HpaA (rHpaA) and rOmp18 were incubated with human peripheral blood mononuclear cells and induced secretion of interleukin-12 (IL-12) and IL-10 from these cells. To determine the effect on antigen-presenting cells, human blood monocytic and dendritic cells (DCs) were isolated by magnetic cell separation. rOmp18 and rHpaA strongly stimulated major histocompatibility class II and CD83 expression 7- to 10-fold on isolated DCs. rHpaA and rOmp18 failed to stimulate IL-8 secretion from monocytes but increased secretion of IL-12 and IL-10 from DCs significantly. In summary, HpaA and Omp18 are recognized by human dendritic cells and induce their maturation as well as antigen presentation. HpaA and Omp18 of H. pylori thereby appear to have a specific antigenic potential in humans.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/12819067-10334982, http://linkedlifedata.com/resource/pubmed/commentcorrection/12819067-10639463, http://linkedlifedata.com/resource/pubmed/commentcorrection/12819067-10731605, http://linkedlifedata.com/resource/pubmed/commentcorrection/12819067-10837075, http://linkedlifedata.com/resource/pubmed/commentcorrection/12819067-10858249, http://linkedlifedata.com/resource/pubmed/commentcorrection/12819067-10878379, http://linkedlifedata.com/resource/pubmed/commentcorrection/12819067-10973276, http://linkedlifedata.com/resource/pubmed/commentcorrection/12819067-10974152, http://linkedlifedata.com/resource/pubmed/commentcorrection/12819067-11101872, http://linkedlifedata.com/resource/pubmed/commentcorrection/12819067-11244046, http://linkedlifedata.com/resource/pubmed/commentcorrection/12819067-11452116, http://linkedlifedata.com/resource/pubmed/commentcorrection/12819067-11642602, http://linkedlifedata.com/resource/pubmed/commentcorrection/12819067-11710844, http://linkedlifedata.com/resource/pubmed/commentcorrection/12819067-11876708, http://linkedlifedata.com/resource/pubmed/commentcorrection/12819067-11913066, http://linkedlifedata.com/resource/pubmed/commentcorrection/12819067-11921447, http://linkedlifedata.com/resource/pubmed/commentcorrection/12819067-12467245, http://linkedlifedata.com/resource/pubmed/commentcorrection/12819067-1827484, http://linkedlifedata.com/resource/pubmed/commentcorrection/12819067-1940369, http://linkedlifedata.com/resource/pubmed/commentcorrection/12819067-2459065, http://linkedlifedata.com/resource/pubmed/commentcorrection/12819067-7592366, http://linkedlifedata.com/resource/pubmed/commentcorrection/12819067-7678592, http://linkedlifedata.com/resource/pubmed/commentcorrection/12819067-8096238, http://linkedlifedata.com/resource/pubmed/commentcorrection/12819067-8613402, http://linkedlifedata.com/resource/pubmed/commentcorrection/12819067-8993017, http://linkedlifedata.com/resource/pubmed/commentcorrection/12819067-9287032, http://linkedlifedata.com/resource/pubmed/commentcorrection/12819067-9316739, http://linkedlifedata.com/resource/pubmed/commentcorrection/12819067-9317031, http://linkedlifedata.com/resource/pubmed/commentcorrection/12819067-9584144, http://linkedlifedata.com/resource/pubmed/commentcorrection/12819067-9597139
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0019-9567
pubmed:author
pubmed:issnType
Print
pubmed:volume
71
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
3837-43
pubmed:dateRevised
2010-9-14
pubmed:meshHeading
pubmed:year
2003
pubmed:articleTitle
Antigenic properties of HpaA and Omp18, two outer membrane proteins of Helicobacter pylori.
pubmed:affiliation
Department of Medicine and Gastroenterology, Technical University of Munich, Munich, Germany.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't