Linked Life Data

12815036

Source:http://linkedlifedata.com/resource/pubmed/id/12815036

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
389
pubmed:dateCreated
2003-7-18
pubmed:abstractText
Higher plants respond to cadmium exposure with the production of phytochelatins (PCn), small heavy metal binding peptides, which are synthesized from glutathione by phytochelatin synthase (PCS). The isolation of a PCS cDNA clone from Brassica juncea L. cv. Vitasso, a candidate species for phytoremediation, is reported here. CLUSTAL analysis revealed a close relationship of BjPCS1 with PCS proteins from Arabidopsis thaliana and Thlaspi caerulescens. BjPCS1 expressed as recombinant protein in E. coli had PCS activity in vitro that was activated by 50 microM Cu and 200 microM Cd to a similar extent. Immunoblot analysis with an antiserum directed against recombinant BjPCS1 showed constitutive PCS expression during plant development. As a percentage of the total protein, the expression was higher in the roots, internodes and petioles in comparison with the leaf tissue. When B. juncea plants were treated with 25 microM cadmium, PCn accumulated increasingly over a 6 d period. Levels in shoots were about 3-fold higher than in roots. Prolonged cadmium exposure caused a significant increase of PCS protein in leaves, whereas in roots PCS protein levels were not affected.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0022-0957
pubmed:author
pubmed:issnType
Print
pubmed:volume
54
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1833-9
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed:year
2003
pubmed:articleTitle
Phytochelatin synthase (PCS) protein is induced in Brassica juncea leaves after prolonged Cd exposure.
pubmed:affiliation
Heidelberger Institut für Pflanzenwissenschaften, INF 360, D-69120 Heidelberg, Germany.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't