Source:http://linkedlifedata.com/resource/pubmed/id/12814990
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
6
|
| pubmed:dateCreated |
2003-6-19
|
| pubmed:abstractText |
Studies investigating human papillomavirus (HPV) viral load as a risk factor in the development of squamous intraepithelial lesions (SILs) and cancer have often yielded conflicting results. These studies used a variety of HPV viral quantitation assays [including the commercially available hybrid capture 2 (HC 2) assay], which differ in their ability to account for differences in cervical cell collection, linear dynamic range of viral load quantitation, and determination of type-specific versus cumulative viral load measures. HPV-16 and HPV-18 viral quantitation using real-time PCR assays were performed to determine whether type-specific viral load measurements that adjust for specimen cellularity result in a different association between viral load and prevalent SIL and cancer, compared with HC 2 quantitation (which does not adjust for cellularity or multiple infections). In general, HPV-16 viral load as measured by real-time PCR increased linearly with increasing grade of SIL while HPV-18 measured using similar techniques increased through low-grade SIL (LSIL), with HPV-18 viral load among high-grade SIL and cancers near the level of cytologically normal women. HC 2 viral load, using the clinical 1.0 pg/ml cut point, differentiated cytologically normal women from women with any level of cytological abnormality (normal versus >/=LSIL) but did not change as lesion severity increased. There was no evidence for plateau of HC 2 at high copy numbers, nor was significant variability in total specimen cellularity observed. However, cumulative viral load measurements by HC 2, in the presence of multiple coinfections, overestimated type-specific viral load. Multiple infections were more common among women with no (32%) or LSIL (51%) [versus 23% in high-grade SIL/cancer], partially explaining the lack of a dose response using a cumulative HC2 viral load measure. The nonrandom distribution of multiple infections by case-control status and the apparent differential effect of viral load by genotype warrant caution when using HC 2 measurements to infer viral load associations with SIL and cancer.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jun
|
| pubmed:issn |
1055-9965
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| pubmed:author |
pubmed-author:BrattiMaria ConcepcionMC,
pubmed-author:BurkRobert DRD,
pubmed-author:GravittPatti EPE,
pubmed-author:HelzlsouerKathy JKJ,
pubmed-author:HerreroRolandoR,
pubmed-author:HildesheimAllanA,
pubmed-author:LorinczAttilaA,
pubmed-author:RodriguezAna CeciliaAC,
pubmed-author:SchiffmanMarkM,
pubmed-author:ShermanMark EME
|
| pubmed:issnType |
Print
|
| pubmed:volume |
12
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
477-84
|
| pubmed:dateRevised |
2007-11-15
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| pubmed:meshHeading |
pubmed-meshheading:12814990-Carcinoma, Squamous Cell,
pubmed-meshheading:12814990-Case-Control Studies,
pubmed-meshheading:12814990-Cervical Intraepithelial Neoplasia,
pubmed-meshheading:12814990-Cohort Studies,
pubmed-meshheading:12814990-Computer Systems,
pubmed-meshheading:12814990-Costa Rica,
pubmed-meshheading:12814990-Cross-Sectional Studies,
pubmed-meshheading:12814990-DNA, Viral,
pubmed-meshheading:12814990-Female,
pubmed-meshheading:12814990-Genotype,
pubmed-meshheading:12814990-Humans,
pubmed-meshheading:12814990-Nucleic Acid Hybridization,
pubmed-meshheading:12814990-Oncogene Proteins, Viral,
pubmed-meshheading:12814990-Papillomaviridae,
pubmed-meshheading:12814990-Polymerase Chain Reaction,
pubmed-meshheading:12814990-Predictive Value of Tests,
pubmed-meshheading:12814990-Prevalence,
pubmed-meshheading:12814990-Severity of Illness Index,
pubmed-meshheading:12814990-Statistics as Topic,
pubmed-meshheading:12814990-Tumor Virus Infections,
pubmed-meshheading:12814990-Uterine Cervical Neoplasms,
pubmed-meshheading:12814990-Viral Load,
pubmed-meshheading:12814990-Women's Health
|
| pubmed:year |
2003
|
| pubmed:articleTitle |
A comparison between real-time polymerase chain reaction and hybrid capture 2 for human papillomavirus DNA quantitation.
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| pubmed:affiliation |
Division of Cancer Epidemiology and Genetics, National Cancer Institute, Bethesda, Maryland 20892, USA. pgravitt@jhsph.edu
|
| pubmed:publicationType |
Journal Article,
Comparative Study,
Evaluation Studies
|