Linked Life Data

12804782

Source:http://linkedlifedata.com/resource/pubmed/id/12804782

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2-3
pubmed:dateCreated
2003-6-13
pubmed:abstractText
We previously reported that the metabotropic glutamate receptor1alpha (mGluR1alpha) has a sensitivity to extracellular polyvalent cations such as Ca(2+) and Gd(3+) as well as glutamate. Gd(3+) binding site was recently identified by crystal structure analysis at the interface of two subunits including Glu238, but it remains unknown whether this site is functionally involved in the activation of mGluR1alpha by Gd(3+) or not. We analyzed the ligand sensitivity of the Glu238Gln mutant, and observed that the sensitivity to extracellular Gd(3+) was completely lost, while the sensitivity to glutamate and Ca(2+) was not affected. We also observed that the presence of Gd(3+) increased the sensitivity of mGluR1alpha to glutamate, and that this effect was again lost by Glu238Gln mutation. These results suggest that the binding of Gd(3+) or a related endogenous substance to this site, alone or in cooperation with glutamate binding at a distant site, leads mGluR1alpha to activation.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0014-5793
pubmed:author
pubmed:issnType
Print
pubmed:day
19
pubmed:volume
545
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
233-8
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
2003
pubmed:articleTitle
Functional identification of Gd3+ binding site of metabotropic glutamate receptor 1alpha.
pubmed:affiliation
Department of Physiology and Cell Biology, Tokyo Medical and Dental University, Graduate School and Faculty of Medicine, D-566, 1-5-45 Yushima, Bunkyo, Tokyo 113-8519, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't