Linked Life Data

12799076

Source:http://linkedlifedata.com/resource/pubmed/id/12799076

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2003-6-11
pubmed:abstractText
Pattern formation in the mouse preimplantation embryo is tightly regulated and essential for successful development. Wnt genes are known to regulate cell interactions and cell fate in invertebrates and vertebrates and, therefore, may play a role in the specification of cell lineages and cellular interactions that occur in preimplantation development. Using degenerate primers based on conserved protein sequences in Wnt coding regions, we have found evidence for Wnt gene expression at the blastocyst stage of mouse preimplantation development. We have identified sequences encoding Wnts3a and 4 and confirmed that these are present as transcripts in early development by using reverse transcriptase-polymerase chain reaction (RT-PCR) with specific primers located in the 5' half of these Wnt genes. Studies on the timing of expression showed that Wnt3a transcripts were present in 2-cell embryos which may represent maternally or embryonically derived transcripts since the major transition of maternal to zygotic gene expression occurs during the late 2-cell stage. Both Wnt3a and 4 transcripts were detected in some precompact 4/8-cell stages with consistent expression detected in all compact 8-, 16-cell and blastocyst stages. To our knowledge, expression of Wnt genes has not been previously described at such an early stage of mammalian development.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
1567-133X
pubmed:author
pubmed:issnType
Print
pubmed:volume
3
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
309-12
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
2003
pubmed:articleTitle
Expression of Wnt genes during mouse preimplantation development.
pubmed:affiliation
School of Medicine, Mailpoint 813, Southampton General Hospital, SO16 6YD Southampton, UK.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't