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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
22
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pubmed:dateCreated |
1992-12-23
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pubmed:abstractText |
As a step toward developing recombinant oral vaccines, we have explored the feasibility of expression of O polysaccharide antigens from Pseudomonas aeruginosa by Escherichia coli. We cloned in E. coli HB101 a 26.2-kilobase DNA fragment from P. aeruginosa strain PA103 that specifies the production of the O polysaccharide of Fisher immunotype 2 (IT-2) strains. The recombinant organism incorporated the P. aeruginosa IT-2 O polysaccharide onto the core of the E. coli lipopolysaccharide (LPS). Transfer of the recombinant plasmid to three LPS-rough strains of P. aeruginosa resulted in synthesis of IT-2 O antigen, and two of these transconjugant strains also synthesized a second O polysaccharide, presumably representing expression of a repressed, or an incomplete, set of genes for an endogenous O polysaccharide. Rabbits injected with the purified recombinant LPS made antibody specific for P. aeruginosa IT-2 O side chains, as did mice fed the recombinant E. coli strain. Expression of P. aeruginosa O antigens by enteric bacteria makes it possible to study these recombinant strains as oral vaccines to prevent P. aeruginosa infections.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/1279692-103840,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1279692-16557868,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1279692-1698385,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1279692-1716810,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1279692-1730487,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1279692-1759494,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1279692-1908162,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1279692-2116444,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1279692-2123852,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1279692-2194094,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1279692-2419563,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1279692-3070321,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1279692-3134788,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1279692-3136157,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1279692-3142017,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1279692-3312013,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1279692-3316029,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1279692-388356,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1279692-3924827,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1279692-4200591,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1279692-4213568,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1279692-429553,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1279692-4626510,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1279692-4896022,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1279692-4977486,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1279692-6187729,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1279692-6290332,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1279692-6378905,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1279692-6404634,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1279692-6405665,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1279692-6413410,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1279692-6424501,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1279692-6425224,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1279692-6427188,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1279692-6432921,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1279692-6798136
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
89
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
10716-20
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pubmed:dateRevised |
2010-9-7
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pubmed:meshHeading |
pubmed-meshheading:1279692-Animals,
pubmed-meshheading:1279692-Antibody Formation,
pubmed-meshheading:1279692-Cloning, Molecular,
pubmed-meshheading:1279692-Conjugation, Genetic,
pubmed-meshheading:1279692-DNA, Bacterial,
pubmed-meshheading:1279692-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:1279692-Escherichia coli,
pubmed-meshheading:1279692-Female,
pubmed-meshheading:1279692-Immunoglobulin A,
pubmed-meshheading:1279692-Immunoglobulin G,
pubmed-meshheading:1279692-Lipopolysaccharides,
pubmed-meshheading:1279692-Mice,
pubmed-meshheading:1279692-O Antigens,
pubmed-meshheading:1279692-Plasmids,
pubmed-meshheading:1279692-Polysaccharides, Bacterial,
pubmed-meshheading:1279692-Pseudomonas aeruginosa
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pubmed:year |
1992
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pubmed:articleTitle |
Cloning and surface expression of Pseudomonas aeruginosa O antigen in Escherichia coli.
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pubmed:affiliation |
Channing Laboratory, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115-5899.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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