Linked Life Data

12788768

Source:http://linkedlifedata.com/resource/pubmed/id/12788768

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
2003-6-5
pubmed:abstractText
An assay to detect UO(2)(2+) complexation was developed based on the chrome azurol S (CAS) assay for siderophores (B. Schwyn and J. B. Neilands, Anal. Biochem. 160:47-56, 1987) and was used to investigate the ability of fungal metabolites to complex actinides. In this assay the discoloration of two dyed agars (one containing a CAS-Fe(3+) dye and the other containing a CAS-UO(2)(2+) dye) caused by ligands was quantified. The assay was tested by using the siderophore desferrioxamine B (DFO), and the results showed that there was a regular, reproducible relationship between discoloration and the amount of siderophore added. The ratio of the discoloration on the CAS-UO(2)(2+) agar to the discoloration on the CAS-Fe(3+) agar was independent of the amount of siderophore added. A total of 113 fungi and yeasts were isolated from three soil samples taken from the Peak District National Park. The fungi were screened for the production of UO(2)(2+) chelators by using the CAS-based assay and were also tested specifically for hydroxamate siderophore production by using the hydroxamate siderophore auxotroph Aureobacterium flavescens JG-9. This organism is highly sensitive to the presence of hydroxamate siderophores. However, the CAS-based assay was found to be less sensitive than the A. flavescens JG-9 assay. No significant difference between the results for each site for the two tests was found. Three isolates were selected for further study and were identified as two Pencillium species and a Mucor species. Our results show that the new assay can be effectively used to screen fungi for the production of UO(2)(2+) chelating ligands. We suggest that hydroxamate siderophores can be produced by mucoraceous fungi.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/12788768-10919769, http://linkedlifedata.com/resource/pubmed/commentcorrection/12788768-11478246, http://linkedlifedata.com/resource/pubmed/commentcorrection/12788768-2952030, http://linkedlifedata.com/resource/pubmed/commentcorrection/12788768-6089517, http://linkedlifedata.com/resource/pubmed/commentcorrection/12788768-6221998, http://linkedlifedata.com/resource/pubmed/commentcorrection/12788768-6242827, http://linkedlifedata.com/resource/pubmed/commentcorrection/12788768-6455965, http://linkedlifedata.com/resource/pubmed/commentcorrection/12788768-6461636, http://linkedlifedata.com/resource/pubmed/commentcorrection/12788768-8057905, http://linkedlifedata.com/resource/pubmed/commentcorrection/12788768-8748280, http://linkedlifedata.com/resource/pubmed/commentcorrection/12788768-9022352, http://linkedlifedata.com/resource/pubmed/commentcorrection/12788768-9299717, http://linkedlifedata.com/resource/pubmed/commentcorrection/12788768-949471
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0099-2240
pubmed:author
pubmed:issnType
Print
pubmed:volume
69
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
3600-6
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
2003
pubmed:articleTitle
Development and application of an assay for uranyl complexation by fungal metabolites, including siderophores.
pubmed:affiliation
Centre for Radiochemistry Research, Department of Chemistry, The University of Manchester, Manchester M13 9PT, United Kingdom. joanna.c.renshaw@man.ac.uk
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't, Evaluation Studies