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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
12
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pubmed:dateCreated |
2003-6-5
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pubmed:abstractText |
The human oncogene bcl-2 exerts protective functions in numerous models of apoptotic cell death and increased oxidative stress. We investigated the effects of inducible bcl-2 overexpression on cellular survival and redox status in dopaminergic rat pheochromocytoma PC 12 cells. Induction of high-level expression of bcl-2 in PC 12 cells resulted in generation of oxidative stress and cessation of growth by cell cycle arrest. Cell cycle arrest in bcl-2-overexpressing PC 12 cells was prevented by an inhibitor of extracellular signal-related kinase (ERK 1/2) activation. Protective effects of bcl-2 expression against L-DOPA neurotoxicity decreased with increasing amounts of bcl-2. Furthermore, high-level bcl-2 overexpression sensitized cells towards oxidative stress and glutathione depletion. Our data suggest that bcl-2 expression is beneficial only in a limited gene dosage range and that high-level expression of bcl-2 exerts potential deleterious effects.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antioxidants,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium-Calmodulin-Dependent...,
http://linkedlifedata.com/resource/pubmed/chemical/Dopamine Agents,
http://linkedlifedata.com/resource/pubmed/chemical/Doxycycline,
http://linkedlifedata.com/resource/pubmed/chemical/Enzyme Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/Flavonoids,
http://linkedlifedata.com/resource/pubmed/chemical/Glutathione,
http://linkedlifedata.com/resource/pubmed/chemical/Levodopa,
http://linkedlifedata.com/resource/pubmed/chemical/Mitogen-Activated Protein Kinase 1,
http://linkedlifedata.com/resource/pubmed/chemical/Mitogen-Activated Protein Kinase 3,
http://linkedlifedata.com/resource/pubmed/chemical/Mitogen-Activated Protein Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/PD 98059,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins c-bcl-2
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0891-5849
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
34
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1517-30
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:12788472-Animals,
pubmed-meshheading:12788472-Antioxidants,
pubmed-meshheading:12788472-Calcium-Calmodulin-Dependent Protein Kinases,
pubmed-meshheading:12788472-Cell Survival,
pubmed-meshheading:12788472-Dopamine Agents,
pubmed-meshheading:12788472-Doxycycline,
pubmed-meshheading:12788472-Enzyme Inhibitors,
pubmed-meshheading:12788472-Flavonoids,
pubmed-meshheading:12788472-Gene Dosage,
pubmed-meshheading:12788472-Gene Expression Regulation,
pubmed-meshheading:12788472-Glutathione,
pubmed-meshheading:12788472-Levodopa,
pubmed-meshheading:12788472-Mitogen-Activated Protein Kinase 1,
pubmed-meshheading:12788472-Mitogen-Activated Protein Kinase 3,
pubmed-meshheading:12788472-Mitogen-Activated Protein Kinases,
pubmed-meshheading:12788472-Oxidation-Reduction,
pubmed-meshheading:12788472-Oxidative Stress,
pubmed-meshheading:12788472-PC12 Cells,
pubmed-meshheading:12788472-Phosphorylation,
pubmed-meshheading:12788472-Proto-Oncogene Proteins c-bcl-2,
pubmed-meshheading:12788472-Rats
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pubmed:year |
2003
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pubmed:articleTitle |
Gene dosage-dependent effects of bcl-2 expression on cellular survival and redox status.
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pubmed:affiliation |
Department of Neurology, University of Bonn, Bonn, Germany. jan.seyfried@man.ac.uk
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
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