Linked Life Data

12767050

Source:http://linkedlifedata.com/resource/pubmed/id/12767050

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2003-5-26
pubmed:abstractText
We tested the expression of genes coding receptors of a cutaneous serotoninergic/melatoninergic system in whole human skin and in normal and pathologic cultured skin cells. Evaluation of serotonin (5HT), melatonin (MT), and melatonin-related receptors (MRR) showed expression of the isoforms 5HT2B, 5HT7, and MT1 genes in almost all the tested samples. Expression of other isoforms was less prevalent; 5HT2C, MRR, and MT2 were rarely detected. We also found novel isoforms for MT2, MRR, and 5HT2B and documented the process of RNA editing for 5HT2C. Testing for functional activity of these receptors with serotonin and melatonin (10(-14) to 10(-10) M) showed variable effects depending on cell type and culture conditions. Thus, serotonin stimulated proliferation of melanocytes in medium deprived of growth factors, while inhibiting cell growth in the presence of growth factors. Melatonin inhibited both apoptosis of HaCaT keratinocytes incubated in serum-free media, and proliferation of cells cultured in medium supplemented with serum. Melatonin also increased the numbers of viable fibroblasts incubated in serum free medium. N-acetylserotonin (NAS) and 5 methoxytryptamine (5MTT) were generally without effect on cell proliferation, with the exception of an inhibition of melanocyte proliferation at the higher 5MTT concentration of 10(-10) M. Thus, skin cells represent a true target for the products of the serotoninergic/melatoninergic cutaneous pathway with their actions modulating cell proliferation or viability.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0021-9541
pubmed:author
pubmed:copyrightInfo
Copyright 2003 Wiley-Liss, Inc.
pubmed:issnType
Print
pubmed:volume
196
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
144-53
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:12767050-5-Methoxytryptamine, pubmed-meshheading:12767050-Amino Acid Sequence, pubmed-meshheading:12767050-Apoptosis, pubmed-meshheading:12767050-Base Sequence, pubmed-meshheading:12767050-Cell Division, pubmed-meshheading:12767050-Cells, Cultured, pubmed-meshheading:12767050-Gene Expression, pubmed-meshheading:12767050-Humans, pubmed-meshheading:12767050-Melatonin, pubmed-meshheading:12767050-Phenotype, pubmed-meshheading:12767050-Pituitary Gland, pubmed-meshheading:12767050-Protein Isoforms, pubmed-meshheading:12767050-RNA, Messenger, pubmed-meshheading:12767050-Receptors, Cell Surface, pubmed-meshheading:12767050-Receptors, Cytoplasmic and Nuclear, pubmed-meshheading:12767050-Receptors, Melatonin, pubmed-meshheading:12767050-Receptors, Serotonin, pubmed-meshheading:12767050-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:12767050-Serotonin, pubmed-meshheading:12767050-Skin
pubmed:year
2003
pubmed:articleTitle
Functional activity of serotoninergic and melatoninergic systems expressed in the skin.
pubmed:affiliation
Department of Pathology, University of Tennessee Health Science Center, Memphis, Tennessee, USA. aslominski@utmem.edu
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't