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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1-2
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pubmed:dateCreated |
2003-5-23
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pubmed:abstractText |
Somatostatin receptors are members of the G-protein-coupled receptor superfamily and exert their principal effects by coupling to inhibitory G-proteins. We used fura-2-based digital calcium imaging and assayed for [3H]inositol phosphates (IPs) to study the effects of somatostatin on intracellular calcium signaling in neuroblastomaxglioma NG108-15 cells. Both somatostatin-14 and octreotide induced concentration-dependent increases in intracellular Ca(2+) concentration ([Ca(2+)](i)). Thirty-four percent of the cells responded to treatment with 100 nM somatostatin-14. Somatostatin-induced responses were not blocked by the removal of extracellular calcium; instead, they were abolished by pretreatment with 100 nM thapsigargin, an agent that depletes and prevents refilling of intracellular Ca(2+) stores. Pretreatment with the inositol 1,4,5-trisphosphate (IP(3)) receptor antagonist xestospongin C (10 microM) for 20 min inhibited markedly the somatostatin-induced response. Somatostatin (100 nM) increased [3H]IPs formation. U73122 (1 microM), an inhibitor of phospholipase C (PLC), completely blocked the somatostatin-induced [Ca(2+)](i) increases and the formation of [3H]IPs. Pretreatment with pertussis toxin (PTX, 200 ng/ml) for 24 h blocked the somatostatin-induced responses. Thus, we conclude that activation of endogenous somatostatin receptors in NG108-15 cells induces the release of calcium from IP(3)-sensitive intracellular stores through PTX-sensitive G-protein-coupled PLC.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/1-(6-((3-methoxyestra-1,3,5(10)-trie...,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium,
http://linkedlifedata.com/resource/pubmed/chemical/Estrenes,
http://linkedlifedata.com/resource/pubmed/chemical/GTP-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Inosine Triphosphate,
http://linkedlifedata.com/resource/pubmed/chemical/Macrocyclic Compounds,
http://linkedlifedata.com/resource/pubmed/chemical/Octreotide,
http://linkedlifedata.com/resource/pubmed/chemical/Oxazoles,
http://linkedlifedata.com/resource/pubmed/chemical/Pertussis Toxin,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphodiesterase Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/Pyrrolidinones,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Somatostatin,
http://linkedlifedata.com/resource/pubmed/chemical/Somatostatin,
http://linkedlifedata.com/resource/pubmed/chemical/Type C Phospholipases,
http://linkedlifedata.com/resource/pubmed/chemical/U 73343,
http://linkedlifedata.com/resource/pubmed/chemical/xestospongin A
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0006-8993
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
13
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pubmed:volume |
975
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
120-8
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:12763599-Animals,
pubmed-meshheading:12763599-Calcium,
pubmed-meshheading:12763599-Cell Line,
pubmed-meshheading:12763599-Estrenes,
pubmed-meshheading:12763599-GTP-Binding Proteins,
pubmed-meshheading:12763599-Glioma,
pubmed-meshheading:12763599-Image Processing, Computer-Assisted,
pubmed-meshheading:12763599-Inosine Triphosphate,
pubmed-meshheading:12763599-Macrocyclic Compounds,
pubmed-meshheading:12763599-Mice,
pubmed-meshheading:12763599-Neuroblastoma,
pubmed-meshheading:12763599-Octreotide,
pubmed-meshheading:12763599-Oxazoles,
pubmed-meshheading:12763599-Pertussis Toxin,
pubmed-meshheading:12763599-Phosphodiesterase Inhibitors,
pubmed-meshheading:12763599-Pyrrolidinones,
pubmed-meshheading:12763599-Receptors, Somatostatin,
pubmed-meshheading:12763599-Somatostatin,
pubmed-meshheading:12763599-Tumor Cells, Cultured,
pubmed-meshheading:12763599-Type C Phospholipases
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pubmed:year |
2003
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pubmed:articleTitle |
Endogenous somatostatin receptors mobilize calcium from inositol 1,4,5-trisphosphate-sensitive stores in NG108-15 cells.
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pubmed:affiliation |
Department of Physiology, College of Medicine, The Catholic University of Korea, 505 Banpo-dong, Socho-gu, Seoul 137-701, South Korea.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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