Source:http://linkedlifedata.com/resource/pubmed/id/12757721
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
12
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pubmed:dateCreated |
2003-5-21
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pubmed:abstractText |
In order to determine the importance of the two ester pharmacophores in high affinity, conformationally constrained DAG-lactones (Lac-1-5) as PK-C ligands, we have independently replaced the sn-1 and sn-2 carbonyl esters in these compounds by ketone (2, 10, 11), amide (3, 25-28), and hydroxyl (12, 13) isosteres. Although the ketone analogue of the sn-1 ester (2) exhibited comparable activity to the parent Lac-1 when taking into account the difference in lipophilicities, the other isosteres were significantly poorer PK-C alpha ligands compared to the parent DAG-lactones. This study demonstrates that the ester functionality in DAG-lactone plays an important role in the ligand's capacity to form a strong hydrogen bond with Gly253 at the active site. The discrete K(i) analysis from the sn-1 and sn-2 isosteres further confirms that the DAG-lactones bind preferentially to the C1-domain in the sn-2 binding mode, as previously suggested.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Amides,
http://linkedlifedata.com/resource/pubmed/chemical/Diglycerides,
http://linkedlifedata.com/resource/pubmed/chemical/Esters,
http://linkedlifedata.com/resource/pubmed/chemical/Lactones,
http://linkedlifedata.com/resource/pubmed/chemical/Ligands,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Kinase C,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0968-0896
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
12
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pubmed:volume |
11
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2529-39
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:12757721-Amides,
pubmed-meshheading:12757721-Diglycerides,
pubmed-meshheading:12757721-Esters,
pubmed-meshheading:12757721-Hydrogen Bonding,
pubmed-meshheading:12757721-Isomerism,
pubmed-meshheading:12757721-Lactones,
pubmed-meshheading:12757721-Ligands,
pubmed-meshheading:12757721-Models, Molecular,
pubmed-meshheading:12757721-Molecular Conformation,
pubmed-meshheading:12757721-Protein Kinase C,
pubmed-meshheading:12757721-Recombinant Proteins,
pubmed-meshheading:12757721-Structure-Activity Relationship
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pubmed:year |
2003
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pubmed:articleTitle |
Conformationally constrained analogues of diacylglycerol (DAG). Effect on protein kinase C (PK-C) binding by the isosteric replacement of sn-1 and sn-2 esters in DAG-lactones.
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pubmed:affiliation |
Laboratory of Medicinal Chemistry, College of Pharmacy, Seoul National University, Seoul 151-742, South Korea.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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