Source:http://linkedlifedata.com/resource/pubmed/id/12752659
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
3
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pubmed:dateCreated |
2003-5-19
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pubmed:abstractText |
The nicotinic acetylcholine receptors are ligand-gated ion channels responsible for rapid neurotransmission and are target sites for pesticides in insects. In the honeybee Apis mellifera, pharmacological and electrophysiological studies have shown that different nicotinic acetylcholine receptor subtypes may exist in the brain. Here, we have identified a honeybee cDNA that encodes a 537 amino acid protein with features typical of nicotinic acetylcholine receptor alpha subunit, and sequence homology to human alpha3. In situ hybridization on cryosections shows that the Apisalpha3 mRNA is differently expressed in larvae and adult. In larvae, Apisalpha3 mRNA expression is restricted to the suboesophageal ganglia. In adult, it is further expressed in the optic lobes, the dorsal lobes, the antennal lobes and the calyces of mushroom bodies. Together our results suggest that Apisalpha3 shows a controlled expression pattern during development.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0962-1075
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
12
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
255-62
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:12752659-Amino Acid Sequence,
pubmed-meshheading:12752659-Animals,
pubmed-meshheading:12752659-Base Sequence,
pubmed-meshheading:12752659-Bees,
pubmed-meshheading:12752659-Brain,
pubmed-meshheading:12752659-Cloning, Molecular,
pubmed-meshheading:12752659-DNA,
pubmed-meshheading:12752659-Gene Expression Regulation,
pubmed-meshheading:12752659-In Situ Hybridization,
pubmed-meshheading:12752659-Molecular Sequence Data,
pubmed-meshheading:12752659-Phylogeny,
pubmed-meshheading:12752659-Polymerase Chain Reaction,
pubmed-meshheading:12752659-RNA, Messenger,
pubmed-meshheading:12752659-Receptors, Nicotinic,
pubmed-meshheading:12752659-Sequence Alignment
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pubmed:year |
2003
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pubmed:articleTitle |
Identification and localization of the nicotinic acetylcholine receptor alpha3 mRNA in the brain of the honeybee, Apis mellifera.
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pubmed:affiliation |
Laboratoire de Neurobiologie de l'Insecte E.A. 3037, Toulouse France. steev.thany@libertysurf.fr
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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