Linked Life Data

12736163

Source:http://linkedlifedata.com/resource/pubmed/id/12736163

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2003-8-5
pubmed:abstractText
Transgenic mice overexpressing growth hormone (GH) have been extensively used to study the chronic effects of elevated serum levels of GH. GH is known to have many acute effects in the liver, but little is known about the chronic effects of GH overexpression on hepatic gene expression. Therefore, we used DNA microarray to compare gene expression in livers from bovine GH (bGH)-transgenic mice and littermates. Hepatic expression of peroxisome proliferator-activated receptor-alpha (PPARalpha) and genes involved in fatty acid activation, peroxisomal and mitochondrial beta-oxidation, and production of ketone bodies was decreased. In line with this expression profile, bGH-transgenic mice had a reduced ability to form ketone bodies in both the fed and fasted states. Although the bGH mice were hyperinsulinemic, the expression of sterol regulatory element-binding protein (SREBP)-1 and most lipogenic enzymes regulated by SREBP-1 was reduced, indicating that these mice are different from other insulin-resistant models with respect to expression of SREBP-1 and its downstream genes. This study also provides several candidate genes for the well-known association between elevated GH levels and cardiovascular disease, e.g., decreased expression of scavenger receptor class B type I, hepatic lipase, and serum paraoxonase and increased expression of serum amyloid A-3 protein. We conclude that bGH-transgenic mice display marked changes in hepatic genes coding for metabolic enzymes and suggest that GH directly or indirectly regulates many of these hepatic genes via decreased expression of PPARalpha and SREBP-1.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0193-1849
pubmed:author
pubmed:issnType
Print
pubmed:volume
285
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
E504-11
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:12736163-Animals, pubmed-meshheading:12736163-CCAAT-Enhancer-Binding Proteins, pubmed-meshheading:12736163-Cattle, pubmed-meshheading:12736163-DNA-Binding Proteins, pubmed-meshheading:12736163-Fatty Acids, pubmed-meshheading:12736163-Gene Expression Regulation, Enzymologic, pubmed-meshheading:12736163-Growth Hormone, pubmed-meshheading:12736163-Hyperinsulinism, pubmed-meshheading:12736163-Ketone Bodies, pubmed-meshheading:12736163-Liver, pubmed-meshheading:12736163-Male, pubmed-meshheading:12736163-Mice, pubmed-meshheading:12736163-Mice, Transgenic, pubmed-meshheading:12736163-Oligonucleotide Array Sequence Analysis, pubmed-meshheading:12736163-Receptors, Cytoplasmic and Nuclear, pubmed-meshheading:12736163-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:12736163-Sterol Regulatory Element Binding Protein 1, pubmed-meshheading:12736163-Transcription Factors
pubmed:year
2003
pubmed:articleTitle
Bovine growth hormone-transgenic mice have major alterations in hepatic expression of metabolic genes.
pubmed:affiliation
Department of Physiology, Göteborg University, SE-405 30 Goteborg, Sweden. bob.olsson@medic.gu.se
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't