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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
2003-5-2
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pubmed:abstractText |
During regeneration, retinal ganglion cell axons in fish upregulate a cell surface protein that is recognized by the monoclonal antibody (mAB) M802. M802 antigen appeared to be linked to the intracellular, membrane-associated lipid raft/microdomain proteins reggie-1 and reggie-2 that were previously shown to be reexpressed in axon-regenerating neurons [Development 124 (1997), 577]. Here, we report the isolation of the M802 antigen and its identification as the teleost homolog of mammalian Thy-1. Fish Thy-1 is detected in the same detergent-insoluble lipid raft fractions from a fibroblast cell line and from axon regenerating retinae as reggie-1 and 2. Importantly, mAB M802 coimmunoprecipitates reggie-1 and 2 from this lipid raft fraction, implying that fish Thy-1 and reggies interact. This correlates with their colocalization in growing cell processes after M802 antigen/Thy-1 activation with mAB M802. These findings suggest a role of clustered M802 antigen/Thy-1 in reggie raft microdomains for cell growth and axon regeneration.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
1044-7431
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
22
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
544-54
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:12727449-Animals,
pubmed-meshheading:12727449-Antigens, Thy-1,
pubmed-meshheading:12727449-Axons,
pubmed-meshheading:12727449-Cells, Cultured,
pubmed-meshheading:12727449-Central Nervous System,
pubmed-meshheading:12727449-DNA, Complementary,
pubmed-meshheading:12727449-Fibroblasts,
pubmed-meshheading:12727449-Fish Proteins,
pubmed-meshheading:12727449-Goldfish,
pubmed-meshheading:12727449-Growth Cones,
pubmed-meshheading:12727449-Membrane Microdomains,
pubmed-meshheading:12727449-Membrane Proteins,
pubmed-meshheading:12727449-Molecular Sequence Data,
pubmed-meshheading:12727449-Nerve Regeneration,
pubmed-meshheading:12727449-Nerve Tissue Proteins,
pubmed-meshheading:12727449-Retinal Ganglion Cells,
pubmed-meshheading:12727449-Sequence Homology, Amino Acid,
pubmed-meshheading:12727449-Sequence Homology, Nucleic Acid
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pubmed:year |
2003
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pubmed:articleTitle |
Identification of teleost Thy-1 and association with the microdomain/lipid raft reggie proteins in regenerating CNS axons.
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pubmed:affiliation |
Department of Chemistry, University of Konstanz, 78457 Konstanz, Germany.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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