Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1-2
pubmed:dateCreated
2003-4-22
pubmed:abstractText
Epidermal growth factor (EGF) superfamily of peptide growth factors (EGF-GFs) plays a role in male germ cell development, but the precise function is yet to be defined. The present study shows that EGF-GFs stimulate spermatogonial proliferation in vitro. The EGF-GF ligands, EGF, transforming growth factor-alpha and betacellulin all stimulated DNA synthesis in microdissected stage I segments of rat testis seminiferous tubules in vitro, as revealed by 3H-thymidine incorporation and 5-bromo-2'-deoxyuridine (BrdU) labeling. A fourfold increase over control of BrdU labeled cells, identified as spermatogonia, was seen after treatment with EGF. RT-PCR analysis revealed that the EGF receptors erbB1, erbB2, erbB3 and erbB4 were expressed at all stages of the spermatogenic wave, whereas differential expression was found in isolated Leydig, Sertoli and peritubular cells. The results show that EGF-GFs is spermatogonial growth factor(s) in vitro, although we have not discriminated between a direct action and an indirect effect via somatic cells. We suggest that EGF-GFs is involved in the paracrine control of spermatogenesis in vivo.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
http://linkedlifedata.com/resource/pubmed/chemical/Bromodeoxyuridine, http://linkedlifedata.com/resource/pubmed/chemical/DNA, http://linkedlifedata.com/resource/pubmed/chemical/Epidermal Growth Factor, http://linkedlifedata.com/resource/pubmed/chemical/Intercellular Signaling Peptides..., http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger, http://linkedlifedata.com/resource/pubmed/chemical/Receptor, Epidermal Growth Factor, http://linkedlifedata.com/resource/pubmed/chemical/Receptor, erbB-2, http://linkedlifedata.com/resource/pubmed/chemical/Receptor, erbB-3, http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Thymidine, http://linkedlifedata.com/resource/pubmed/chemical/Transforming Growth Factor alpha, http://linkedlifedata.com/resource/pubmed/chemical/betacellulin, http://linkedlifedata.com/resource/pubmed/chemical/receptor tyrosine-protein kinase...
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0303-7207
pubmed:author
pubmed:issnType
Print
pubmed:day
28
pubmed:volume
201
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
39-46
pubmed:dateRevised
2011-11-2
pubmed:meshHeading
pubmed-meshheading:12706292-Animals, pubmed-meshheading:12706292-Bromodeoxyuridine, pubmed-meshheading:12706292-Cell Division, pubmed-meshheading:12706292-Cells, Cultured, pubmed-meshheading:12706292-DNA, pubmed-meshheading:12706292-Dose-Response Relationship, Drug, pubmed-meshheading:12706292-Epidermal Growth Factor, pubmed-meshheading:12706292-Intercellular Signaling Peptides and Proteins, pubmed-meshheading:12706292-Leydig Cells, pubmed-meshheading:12706292-Male, pubmed-meshheading:12706292-RNA, Messenger, pubmed-meshheading:12706292-Rats, pubmed-meshheading:12706292-Rats, Sprague-Dawley, pubmed-meshheading:12706292-Receptor, Epidermal Growth Factor, pubmed-meshheading:12706292-Receptor, erbB-2, pubmed-meshheading:12706292-Receptor, erbB-3, pubmed-meshheading:12706292-Recombinant Proteins, pubmed-meshheading:12706292-Seminiferous Tubules, pubmed-meshheading:12706292-Sertoli Cells, pubmed-meshheading:12706292-Spermatogonia, pubmed-meshheading:12706292-Thymidine, pubmed-meshheading:12706292-Transforming Growth Factor alpha
pubmed:year
2003
pubmed:articleTitle
EGF stimulates rat spermatogonial DNA synthesis in seminiferous tubule segments in vitro.
pubmed:affiliation
Pediatric Endocrinology Unit, Q2:08, Department of Woman and Child Health, Astrid Lindgren Children's Hospital, Karolinska Institute and Hospital, S-171 76 Stockholm, Sweden. aida.wahlgren@kbh.ki.se
pubmed:publicationType
Journal Article, In Vitro, Research Support, Non-U.S. Gov't