Source:http://linkedlifedata.com/resource/pubmed/id/12695849
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
2
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pubmed:dateCreated |
2003-4-15
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pubmed:abstractText |
The promoter of avirulence gene Avr9 of the fungal tomato pathogen Cladosporium fulvum contains 12 sequences within a region of 0.6 kb that are reminiscent of the binding sequences of the GATA-type regulator involved in nitrogen utilisation of the filamentous fungi Aspergillus nidulans and Neurospora crassa. Mutational analysis of this 0.6-kb promoter region, fused to the beta-glucuronidase reporter gene, revealed that two regions, each containing two TAGATA boxes in inverted orientation and overlapping by two base pairs, are important for induction of Avr9 promoter activity in A. nidulans. Each overlapping TAGATA box differentially affected Avr9 promoter activity when shifted apart by nucleotide insertions. The other regions, which do not contain two overlapping TAGATA boxes have no, or only a limited, contribution to the inducibility of promoter activity.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0172-8083
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
43
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
96-102
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:12695849-Aspergillus nidulans,
pubmed-meshheading:12695849-Chromosome Mapping,
pubmed-meshheading:12695849-Cladosporium,
pubmed-meshheading:12695849-DNA Mutational Analysis,
pubmed-meshheading:12695849-DNA Primers,
pubmed-meshheading:12695849-Fungal Proteins,
pubmed-meshheading:12695849-Gene Expression Regulation,
pubmed-meshheading:12695849-Genes, Reporter,
pubmed-meshheading:12695849-Glucuronidase,
pubmed-meshheading:12695849-Plasmids,
pubmed-meshheading:12695849-Promoter Regions, Genetic,
pubmed-meshheading:12695849-Transformation, Genetic
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pubmed:year |
2003
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pubmed:articleTitle |
Promoter analysis of the avirulence gene Avr9 of the fungal tomato pathogen Cladosporium fulvum in the model filamentous fungus Aspergillus nidulans.
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pubmed:affiliation |
Laboratory of Phytopathology, Department of Plant Sciences, Wageningen University, Binnenhaven 5, 6709 PD Wageningen, The Netherlands.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
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