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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
8
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pubmed:dateCreated |
2003-4-8
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pubmed:abstractText |
Unmethylated CpG motifs in bacterial DNA or synthetic oligodeoxynucleotides (ODN) are known for inducing a Th1 cytokine/chemokine environment, but the mechanisms regulating this have been unclear. Recent studies have defined two classes of CpG ODN, CpG-A ODN that induce plasmacytoid dendritic cells (pDC) to secrete very high levels of IFN-alpha, and CpG-B ODN that induce only low levels of IFN-alpha production, but strongly activate B cells. We now demonstrate that a CpG-A ODN directly activates pDC secretion of IFN-alpha and other soluble factors that secondarily induce purified monocytes to secrete high levels of the Th1-promoting chemokine IFN-gamma-inducible protein-10 (IP-10). Cell contact between the monocytes and pDC is not required for this interaction. IFN-alpha is necessary, but only partially sufficient, for this indirect CpG-induced monocyte IP-10 production. Although CpG ODN induce human PBMC to make only very slight amounts of IFN-gamma, we find that these low concentrations synergize with IFN-alpha for inducing monocyte production of IP-10. These studies provide a better understanding of the mechanisms through which CpG ODN create a Th1-like environment.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0022-1767
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
170
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
4061-8
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:12682235-B-Lymphocyte Subsets,
pubmed-meshheading:12682235-Cell Communication,
pubmed-meshheading:12682235-Cell Separation,
pubmed-meshheading:12682235-Cells, Cultured,
pubmed-meshheading:12682235-Chemokine CXCL10,
pubmed-meshheading:12682235-Chemokines, CXC,
pubmed-meshheading:12682235-CpG Islands,
pubmed-meshheading:12682235-Dendritic Cells,
pubmed-meshheading:12682235-Drug Synergism,
pubmed-meshheading:12682235-Humans,
pubmed-meshheading:12682235-Immune Sera,
pubmed-meshheading:12682235-Immunophenotyping,
pubmed-meshheading:12682235-Interferon-alpha,
pubmed-meshheading:12682235-Interferon-gamma,
pubmed-meshheading:12682235-Leukocytes, Mononuclear,
pubmed-meshheading:12682235-Monocytes,
pubmed-meshheading:12682235-Oligodeoxyribonucleotides,
pubmed-meshheading:12682235-Plasma Cells,
pubmed-meshheading:12682235-T-Lymphocyte Subsets
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pubmed:year |
2003
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pubmed:articleTitle |
CpG-A-induced monocyte IFN-gamma-inducible protein-10 production is regulated by plasmacytoid dendritic cell-derived IFN-alpha.
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pubmed:affiliation |
Department of Internal Medicine, University of Iowa, Iowa City, IA 52242, USA.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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