Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
8
pubmed:dateCreated
2003-4-16
pubmed:abstractText
Whereas information is rapidly accumulating about the structure and position of genes encoded in the human genome, less is known about the complexity and relative abundance of their expression in individual human cells and tissues. Here, we describe the characteristics of the transcriptomes of two cultured cell lines, HB4a (normal breast epithelium) and HCT-116 (colon adenocarcinoma), using massively parallel signature sequencing (MPSS). We generated in excess of 10(7) short signature sequences per cell line, thus providing a comprehensive snapshot of gene expression, within the technical limitations of the method. The number of genes expressed at one copy per cell or more in either of the lines was estimated to be between 10,000 and 15,000. The vast majority of the transcripts found in these cells can be mapped to known genes and their polyadenylation variants. Among the genes that could be identified from their signature sequences, approximately 8,500 were expressed by both cell lines, whereas 6,000 showed cellular specificity. Taking into account sequence tags that map uniquely to the genome but not to known transcripts, overall the data are consistent with an upper limit of 17,000 for the total number of genes expressed at more than one copy per cell in one or both of the two cell lines examined.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/12671075-1017013, http://linkedlifedata.com/resource/pubmed/commentcorrection/12671075-10554005, http://linkedlifedata.com/resource/pubmed/commentcorrection/12671075-10581018, http://linkedlifedata.com/resource/pubmed/commentcorrection/12671075-10677516, http://linkedlifedata.com/resource/pubmed/commentcorrection/12671075-10835600, http://linkedlifedata.com/resource/pubmed/commentcorrection/12671075-11292841, http://linkedlifedata.com/resource/pubmed/commentcorrection/12671075-11454705, http://linkedlifedata.com/resource/pubmed/commentcorrection/12671075-11592992, http://linkedlifedata.com/resource/pubmed/commentcorrection/12671075-11593022, http://linkedlifedata.com/resource/pubmed/commentcorrection/12671075-12097343, http://linkedlifedata.com/resource/pubmed/commentcorrection/12671075-12119410, http://linkedlifedata.com/resource/pubmed/commentcorrection/12671075-12213963, http://linkedlifedata.com/resource/pubmed/commentcorrection/12671075-12449737, http://linkedlifedata.com/resource/pubmed/commentcorrection/12671075-4855195, http://linkedlifedata.com/resource/pubmed/commentcorrection/12671075-7214343, http://linkedlifedata.com/resource/pubmed/commentcorrection/12671075-7570003, http://linkedlifedata.com/resource/pubmed/commentcorrection/12671075-7640223, http://linkedlifedata.com/resource/pubmed/commentcorrection/12671075-8401585
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0027-8424
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
100
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
4702-5
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
2003
pubmed:articleTitle
Comprehensive sampling of gene expression in human cell lines with massively parallel signature sequencing.
pubmed:affiliation
Office of Information Technology, Ludwig Institute for Cancer Research, and Swiss Institute of Bioinformatics, 1066 Epalinges, Switzerland. Victor.Jongeneel@licr.ch
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't