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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
14
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pubmed:dateCreated |
2003-4-2
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pubmed:databankReference |
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pubmed:abstractText |
Protein tyrosine phosphatase 1B (PTP1B) is an enzyme that downregulates the insulin receptor. Inhibition of PTP1B is expected to improve insulin action, and the design of small molecule PTP1B inhibitors to treat type II diabetes has received considerable attention. In this work, NMR-based screening identified a nonselective competitive inhibitor of PTP1B. A second site ligand was also identified by NMR-based screening and then linked to the catalytic site ligand by rational design. X-ray data confirmed that the inhibitor bound with the catalytic site in the native, "open" conformation. The final compound displayed excellent potency and good selectivity over many other phosphatases. The modular approach to drug design described in this work should be applicable for the design of potent and selective inhibitors of other therapeutically relevant protein tyrosine phosphatases.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0002-7863
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pubmed:author |
pubmed-author:Abad-ZapateroCeleC,
pubmed-author:BallaronStephen JSJ,
pubmed-author:DemeLL,
pubmed-author:FesikStephen WSW,
pubmed-author:HajdukPhilip JPJ,
pubmed-author:HutchinsCharles WCW,
pubmed-author:JirousekMichael RMR,
pubmed-author:LiangHengH,
pubmed-author:LiuGangG,
pubmed-author:LubbenThomas HTH,
pubmed-author:PeiZhonghuaZ,
pubmed-author:StashkoMichael AMA,
pubmed-author:SzczepankiewiczBruce GBG,
pubmed-author:TrevillyanJames MJM,
pubmed-author:XinZhiliZ
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pubmed:issnType |
Print
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pubmed:day |
9
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pubmed:volume |
125
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
4087-96
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:12670229-Amino Acid Sequence,
pubmed-meshheading:12670229-Binding, Competitive,
pubmed-meshheading:12670229-Binding Sites,
pubmed-meshheading:12670229-Catalysis,
pubmed-meshheading:12670229-Drug Design,
pubmed-meshheading:12670229-Enzyme Inhibitors,
pubmed-meshheading:12670229-Kinetics,
pubmed-meshheading:12670229-Ligands,
pubmed-meshheading:12670229-Models, Molecular,
pubmed-meshheading:12670229-Molecular Sequence Data,
pubmed-meshheading:12670229-Nuclear Magnetic Resonance, Biomolecular,
pubmed-meshheading:12670229-Oxamic Acid,
pubmed-meshheading:12670229-Protein Conformation,
pubmed-meshheading:12670229-Protein Tyrosine Phosphatase, Non-Receptor Type 1,
pubmed-meshheading:12670229-Protein Tyrosine Phosphatases,
pubmed-meshheading:12670229-Structure-Activity Relationship
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pubmed:year |
2003
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pubmed:articleTitle |
Discovery of a potent, selective protein tyrosine phosphatase 1B inhibitor using a linked-fragment strategy.
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pubmed:affiliation |
Metabolic Disease Research, Global Pharmaceutical Research and Development Organization, Abbott Laboratories, 100 Abbott Park Road, Abbott Park, Illinois 60064, USA. bruce.szczepankiewicz@abbott.com
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pubmed:publicationType |
Journal Article
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