Linked Life Data

12666199

Source:http://linkedlifedata.com/resource/pubmed/id/12666199

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2003-3-31
pubmed:abstractText
Proteoglycans are involved in secondary palate formation. In the present study, we focused on two small leucine-rich proteoglycans, decorin and biglycan, because they assembled extracellular matrix molecules such as collagens and modulated signaling pathway of transforming growth factor-beta. To investigate the functions of decorin and biglycan in palatogenesis, we compared their mRNA expression patterns between normal palate and retinoic acid-induced cleft palate in mice by using in situ hybridization analysis during the period of embryonic day 13.5 (E13.5) to E15.5. On E13.5, decorin mRNA was expressed in the epithelia and mesenchyme on the nasal side of the developing secondary palate. During the period the palate shelves were fusing (E14.5), decorin mRNA was strongly expressed in the mesenchyme but its expression pattern was asymmetric; decorin mRNA expression area in the nasal side was broader than that in the oral side. The expression of decorin mRNA was hardly detected in the mesenchyme on either side of the medial edge epithelium. After fusion (E15.5), its expression converged to the mesenchyme just around the palatine bone. Biglycan mRNA was ubiquitously distributed throughout the palatal mesenchyme for the mid-gestation period. Its expression area became limited to the ossification area within the palate after the late gestation period. In the retinoic acid-treated mice, the area of the decorin gene expression expanded to the core region of the palate primordium where little signal was observed in control mice. On the other hand, biglycan in the retinoic acid-treated mice did not show remarkable change in its distribution patterns compared with that in the control mice. These findings suggest that decorin and biglycan play distinct roles in palatogenesis, and decorin was more actively involved in the process of secondary palate formation than biglycan. Up-regulation of decorin gene expression in the retinoic acid-treated mice might influence the pathogenesis of cleft palate.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
1058-8388
pubmed:author
pubmed:copyrightInfo
Copyright 2003 Wiley-Liss, Inc.
pubmed:issnType
Print
pubmed:volume
226
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
618-26
pubmed:dateRevised
2010-11-18
pubmed:meshHeading
pubmed-meshheading:12666199-Animals, pubmed-meshheading:12666199-Antineoplastic Agents, pubmed-meshheading:12666199-Biglycan, pubmed-meshheading:12666199-Cell Division, pubmed-meshheading:12666199-Cleft Palate, pubmed-meshheading:12666199-Decorin, pubmed-meshheading:12666199-Extracellular Matrix, pubmed-meshheading:12666199-Extracellular Matrix Proteins, pubmed-meshheading:12666199-Female, pubmed-meshheading:12666199-Gene Expression Regulation, Developmental, pubmed-meshheading:12666199-Male, pubmed-meshheading:12666199-Mesoderm, pubmed-meshheading:12666199-Mice, pubmed-meshheading:12666199-Mice, Inbred ICR, pubmed-meshheading:12666199-Palate, pubmed-meshheading:12666199-Pregnancy, pubmed-meshheading:12666199-Proteoglycans, pubmed-meshheading:12666199-RNA, Messenger, pubmed-meshheading:12666199-Signal Transduction, pubmed-meshheading:12666199-Transforming Growth Factor beta, pubmed-meshheading:12666199-Tretinoin
pubmed:year
2003
pubmed:articleTitle
Differential expression of decorin and biglycan genes during palatogenesis in normal and retinoic acid-treated mice.
pubmed:affiliation
Department of Anesthesiology, School of Medicine, Fukushima Medical University, Fukushima City, Fukushima, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't