Source:http://linkedlifedata.com/resource/pubmed/id/12662302
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
4
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| pubmed:dateCreated |
2003-3-28
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| pubmed:abstractText |
Autoimmune hepatitis (AIH) is characterized by dense T-cell infiltrations in the liver tissue, but little is known how T cells influence the pathogenesis. To address this question, the distribution of T-cell receptor variable beta-chain (TCR Vbeta) transcripts of peripheral blood and liver-infiltrating T cells from previously untreated patients with newly diagnosed acute exacerbated AIH was investigated. Furthermore, the lengths and sequences of complementary-determining region 3 (CDR3) were studied. Reverse transcriptase-polymerase chain reaction (RT-PCR) analysis and CDR3 spectratyping revealed multiple clonal expansions of liver-infiltrating T cells but not peripheral T cells within various TCR Vbeta families. Further analysis of overexpressed TCR Vbeta transcripts using TCR beta-chain-joining element (TCR Jbeta)-specific primers in a nested PCR showed characteristic Vbeta/Jbeta combinations. Subsequent sequencing of CDR3 regions from PCR products confirmed the clonality of T-cell expansions and the usage of common and individual CDR3 motifs. In conclusion, the clonality of expanded T cells within the liver tissue during early clinical manifestation of untreated AIH indicated that autoantigen-specific T cells accumulate at the inflammation site. Individual and common CDR3 motifs argued for predominant epitopes that were recognized by liver-infiltrating T cells in AIH patients.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Complementarity Determining Regions,
http://linkedlifedata.com/resource/pubmed/chemical/Epitopes, T-Lymphocyte,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Antigen, T-Cell
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| pubmed:status |
MEDLINE
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| pubmed:month |
Apr
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| pubmed:issn |
0300-9475
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| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
57
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
384-90
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| pubmed:dateRevised |
2006-11-15
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| pubmed:meshHeading |
pubmed-meshheading:12662302-Adult,
pubmed-meshheading:12662302-Aged,
pubmed-meshheading:12662302-Amino Acid Sequence,
pubmed-meshheading:12662302-Base Sequence,
pubmed-meshheading:12662302-Biopsy,
pubmed-meshheading:12662302-Clone Cells,
pubmed-meshheading:12662302-Complementarity Determining Regions,
pubmed-meshheading:12662302-Epitopes, T-Lymphocyte,
pubmed-meshheading:12662302-Female,
pubmed-meshheading:12662302-Gene Expression Regulation,
pubmed-meshheading:12662302-Hepatitis, Autoimmune,
pubmed-meshheading:12662302-Humans,
pubmed-meshheading:12662302-Male,
pubmed-meshheading:12662302-Middle Aged,
pubmed-meshheading:12662302-Molecular Sequence Data,
pubmed-meshheading:12662302-RNA, Messenger,
pubmed-meshheading:12662302-Receptors, Antigen, T-Cell,
pubmed-meshheading:12662302-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:12662302-T-Lymphocytes
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| pubmed:year |
2003
|
| pubmed:articleTitle |
Individual and common antigen-recognition sites of liver-derived T cells in patients with autoimmune hepatitis.
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| pubmed:affiliation |
Department of Internal Medicine, Johannes-Gutenberg-University Mainz, Langenbeckstrasse 1, 55122 Mainz, Germany. loehr@mail.uni-mainz.de
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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