Source:http://linkedlifedata.com/resource/pubmed/id/12657460
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
4
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| pubmed:dateCreated |
2003-3-26
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| pubmed:abstractText |
Considerable evidence suggests that an N-methyl-D-aspartate (NMDA) receptor plays a crucial role in memory and cognitive function. To identify the role of this receptor in higher functions of the brain, we delivered antisense oligonucleotides against an NMDA-NR1 subunit (NR1) to the hippocampus in rats using the HVJ-liposome-mediated gene-transfer method. NR1 hippocampal knockdown was performed by the focal injection of the NR1 antisense-HVJ-liposome complex into the bilateral hippocampus. The blocking effect of NR1-antisense on the expression of NR1 was confirmed by Western blot analysis. Spatial memory was tested by a water maze task, and sensorimotor gating was examined by prepulse inhibition (PPI). Western blot analysis demonstrated that the NR1-antisense treatment specifically provided the down-regulation (about 30%) of NR1 protein levels in the hippocampus. The water maze task showed that the antisense treatment did not affect spatial memory, while the PPI test revealed that NR1 hippocampal knockdown caused a deficit in sensorimotor gating. We conclude that mild dysfunction of hippocampal NMDA receptor causes sensorimotor gating deficit and relatively intact in spatial memory.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Liposomes,
http://linkedlifedata.com/resource/pubmed/chemical/NMDA receptor A1,
http://linkedlifedata.com/resource/pubmed/chemical/Oligonucleotides, Antisense,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, N-Methyl-D-Aspartate,
http://linkedlifedata.com/resource/pubmed/chemical/Thionucleotides
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| pubmed:status |
MEDLINE
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| pubmed:month |
Apr
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| pubmed:issn |
0168-0102
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| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
45
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
473-81
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| pubmed:dateRevised |
2006-11-15
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| pubmed:meshHeading |
pubmed-meshheading:12657460-Animals,
pubmed-meshheading:12657460-Animals, Genetically Modified,
pubmed-meshheading:12657460-Down-Regulation,
pubmed-meshheading:12657460-Gene Transfer, Horizontal,
pubmed-meshheading:12657460-Gene Transfer Techniques,
pubmed-meshheading:12657460-Genetic Vectors,
pubmed-meshheading:12657460-Hippocampus,
pubmed-meshheading:12657460-Injections, Intraventricular,
pubmed-meshheading:12657460-Liposomes,
pubmed-meshheading:12657460-Male,
pubmed-meshheading:12657460-Maze Learning,
pubmed-meshheading:12657460-Memory,
pubmed-meshheading:12657460-Motor Activity,
pubmed-meshheading:12657460-Oligonucleotides, Antisense,
pubmed-meshheading:12657460-Rats,
pubmed-meshheading:12657460-Rats, Wistar,
pubmed-meshheading:12657460-Receptors, N-Methyl-D-Aspartate,
pubmed-meshheading:12657460-Sendai virus,
pubmed-meshheading:12657460-Thionucleotides
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| pubmed:year |
2003
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| pubmed:articleTitle |
Antisense hippocampal knockdown of NMDA-NR1 by HVJ-liposome vector induces deficit of prepulse inhibition but not of spatial memory.
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| pubmed:affiliation |
Department of Psychiatry, School of Medicine, Kitasato University, Sagamihara, Kanagawa 228-8520, Japan. byi00425@nifty.com
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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