Linked Life Data

12657370

Source:http://linkedlifedata.com/resource/pubmed/id/12657370

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2003-3-26
pubmed:abstractText
The biochemical and physiological processes involved in apoptosis were described from the perspective of detection by standard, clinical, noninvasive imaging modalities. The difficulties of monitoring apoptosis in vivo were discussed. Magnetic resonance imaging (MRI) approaches used to study apoptosis were surveyed. The cell shrinkage associated with apoptosis can be detected due to changes in tissue water T(2) and T(1)rho relaxation times and apparent diffusion coefficient (ADC). Magnetic resonance spectroscopy (MRS) approaches used to study apoptosis in vivo have largely centered on the formation of cytoplasmic lipid bodies, detected by 1H MRS, and metabolic/bioenergetic changes detected by 31P and 13C MRS. The most successful approach to in vivo mapping of apoptosis uses the high specific binding of annexin V or synaptotagmin I to phosphatidylserine (PS) that appears on the extracellular plasma membrane of cells during apoptosis. Technetium-99m (99mTc)-radiolabeling of the annexin V and superparamagnetic iron oxide (SPIO) labeling of the C2 domain of synaptotagmin I allow good in vivo apoptosis detection by gamma camera imaging and MRI, respectively.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0278-5846
pubmed:author
pubmed:issnType
Print
pubmed:volume
27
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
323-31
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
2003
pubmed:articleTitle
In vivo monitoring of apoptosis.
pubmed:affiliation
Department of Chemistry and Biochemistry, University of Guelph, N1G 2W1, Guelph, Ontario, Canada. brauer@chembio.uoguelph.ca
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Review