Linked Life Data

12645905

Source:http://linkedlifedata.com/resource/pubmed/id/12645905

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2003-3-20
pubmed:abstractText
An improved capillary liquid chromatography procedure, incorporating column switching in combination with mass spectrometry, is reported. The dual column system allows for rapid inject-to-inject cycle times to improve the speed of protein identification for proteomics applications. Full gradient elution of peptides from either of the two C18 columns can be achieved in less than 17 min while maintaining sufficient resolution for the peptides to be detected and fragmented by the mass spectrometer for protein identification. Importantly, the use of two columns for subsequent injections is reproducible and without carry-over. The limit of detection for the system is between 25 and 50 fmol per injection. This fully automated system is capable of analyzing and identifying proteins from an entire 96-well plate in about 27 h.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
1535-3893
pubmed:author
pubmed:issnType
Print
pubmed:volume
1
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
279-84
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:articleTitle
Capillary chromatography-coupled mass spectrometry with column switching for rapid identification of proteins from 2-dimensional electrophoresis gels.
pubmed:affiliation
Procter & Gamble Pharmaceuticals, 8700 Mason-Montgomery Road, Mason, Ohio 45040, USA.
pubmed:publicationType
Journal Article, Evaluation Studies