Linked Life Data

12631709

Source:http://linkedlifedata.com/resource/pubmed/id/12631709

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2003-3-12
pubmed:abstractText
The molecular mechanisms of clathrin-dependent internalization of epidermal growth factor receptor (EGFR) are not well understood and, in particular, the sequence motifs that mediate EGFR interactions with coated pits have not been mapped. We generated a panel of EGFR mutants and stably expressed these mutants in porcine aortic endothelial (PAE) cells. Interestingly, mutations of tyrosine phosphorylation sites 1068 and 1086 that interact with growth-factor-receptor-binding protein Grb2 completely abolished receptor internalization in PAE cells. Quantitative analysis of colocalization of EGF-rhodamine conjugate and coated pits labeled with yellow-fluorescent-protein-tagged beta2 subunit of clathrin adaptor complex AP-2 revealed that EGFR mutants lacking Grb2 binding sites do not efficiently enter coated pits. The depletion of Grb2 from PAE as well as HeLa cells expressing endogenous EGFRs by RNA interference substantially reduced the rate of EGFR internalization through clathrin-dependent pathway, thus providing the direct evidence for the important role of Grb2 in this process. Overexpression of Grb2 mutants, in which the SH3 domains were either deleted or inactivated by point mutations, significantly inhibited EGFR internalization in both PAE and HeLa cells. These findings indicate that Grb2, in addition to its key function in signaling through Ras, has a major regulatory role at the initial steps of EGFR internalization through clathrin-coated pits. Furthermore, the EGFR mutant lacking Grb2 binding sites did not efficiently recruit c-Cbl and was not polyubiquitinated. The data are consistent with the model whereby Grb2 participates in EGFR internalization through the recruitment of Cbl to the receptor, thus allowing proper ubiquitylation of EGFR and/or associated proteins at the plasma membrane.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/12631709-10228163, http://linkedlifedata.com/resource/pubmed/commentcorrection/12631709-10635327, http://linkedlifedata.com/resource/pubmed/commentcorrection/12631709-10953014, http://linkedlifedata.com/resource/pubmed/commentcorrection/12631709-11057895, http://linkedlifedata.com/resource/pubmed/commentcorrection/12631709-11373684, http://linkedlifedata.com/resource/pubmed/commentcorrection/12631709-11823423, http://linkedlifedata.com/resource/pubmed/commentcorrection/12631709-11894095, http://linkedlifedata.com/resource/pubmed/commentcorrection/12631709-11956311, http://linkedlifedata.com/resource/pubmed/commentcorrection/12631709-12006650, http://linkedlifedata.com/resource/pubmed/commentcorrection/12631709-12021271, http://linkedlifedata.com/resource/pubmed/commentcorrection/12631709-1314835, http://linkedlifedata.com/resource/pubmed/commentcorrection/12631709-1744139, http://linkedlifedata.com/resource/pubmed/commentcorrection/12631709-1975591, http://linkedlifedata.com/resource/pubmed/commentcorrection/12631709-2022651, http://linkedlifedata.com/resource/pubmed/commentcorrection/12631709-2418017, http://linkedlifedata.com/resource/pubmed/commentcorrection/12631709-2790960, http://linkedlifedata.com/resource/pubmed/commentcorrection/12631709-3262110, http://linkedlifedata.com/resource/pubmed/commentcorrection/12631709-7527043, http://linkedlifedata.com/resource/pubmed/commentcorrection/12631709-7608194, http://linkedlifedata.com/resource/pubmed/commentcorrection/12631709-8396132, http://linkedlifedata.com/resource/pubmed/commentcorrection/12631709-8583923, http://linkedlifedata.com/resource/pubmed/commentcorrection/12631709-8658166, http://linkedlifedata.com/resource/pubmed/commentcorrection/12631709-8662849, http://linkedlifedata.com/resource/pubmed/commentcorrection/12631709-9307968, http://linkedlifedata.com/resource/pubmed/commentcorrection/12631709-9857032, http://linkedlifedata.com/resource/pubmed/commentcorrection/12631709-9865697
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
1059-1524
pubmed:author
pubmed:issnType
Print
pubmed:volume
14
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
858-70
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed-meshheading:12631709-Adaptor Proteins, Signal Transducing, pubmed-meshheading:12631709-Animals, pubmed-meshheading:12631709-Binding Sites, pubmed-meshheading:12631709-Cell Line, pubmed-meshheading:12631709-Clathrin-Coated Vesicles, pubmed-meshheading:12631709-Endocytosis, pubmed-meshheading:12631709-Endothelium, Vascular, pubmed-meshheading:12631709-GRB2 Adaptor Protein, pubmed-meshheading:12631709-Oncogene Protein v-cbl, pubmed-meshheading:12631709-Protein Binding, pubmed-meshheading:12631709-Protein Transport, pubmed-meshheading:12631709-Proteins, pubmed-meshheading:12631709-RNA Interference, pubmed-meshheading:12631709-Receptor, Epidermal Growth Factor, pubmed-meshheading:12631709-Recombinant Fusion Proteins, pubmed-meshheading:12631709-Retroviridae Proteins, Oncogenic, pubmed-meshheading:12631709-Swine, pubmed-meshheading:12631709-Tyrosine
pubmed:year
2003
pubmed:articleTitle
Grb2 regulates internalization of EGF receptors through clathrin-coated pits.
pubmed:affiliation
Department of Pharmacology, University of Colorado Health Sciences Center, Denver 80111, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't