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pubmed:abstractText |
Adrenomedullin is a hypotensive peptide secreted from various cells. Recently, we found that adrenomedullin, but not calcitonin gene-related peptide (CGRP), stimulates histamine release from rat peritoneal mast cells. In the present studies, we investigated the expression of mRNA for calcitonin-receptor-like receptor (CRLR) and receptor-activity modifying proteins (RAMPs), the components of proposed adrenomedullin receptors, in rat peritoneal mast cells by reverse transcription-polymerase chain reaction (RT-PCR). Results revealed that mRNA for CRLR, RAMP2 and RAMP3 was expressed in rat peritoneal mast cells, whereas mRNA for RAMP1 was not. These data suggest that adrenomedullin might stimulate histamine release via its proposed receptor (CRLR/RAMP2 or 3), rather than via the CGRP receptor (CRLR/RAMP1).
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