| pubmed-article:12618403 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:12618403 | lifeskim:mentions | umls-concept:C0021585 | lld:lifeskim |
| pubmed-article:12618403 | lifeskim:mentions | umls-concept:C0205245 | lld:lifeskim |
| pubmed-article:12618403 | lifeskim:mentions | umls-concept:C1511790 | lld:lifeskim |
| pubmed-article:12618403 | lifeskim:mentions | umls-concept:C0079868 | lld:lifeskim |
| pubmed-article:12618403 | lifeskim:mentions | umls-concept:C0887950 | lld:lifeskim |
| pubmed-article:12618403 | lifeskim:mentions | umls-concept:C0086222 | lld:lifeskim |
| pubmed-article:12618403 | lifeskim:mentions | umls-concept:C0336791 | lld:lifeskim |
| pubmed-article:12618403 | lifeskim:mentions | umls-concept:C1705733 | lld:lifeskim |
| pubmed-article:12618403 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:12618403 | pubmed:dateCreated | 2003-3-5 | lld:pubmed |
| pubmed-article:12618403 | pubmed:abstractText | Transposon mutagenesis provides a fundamental tool for functional genomics. Here we present a non-species-specific, combined enhancer detection and binary expression system based on the transposable element piggyBac: For the different components of this insertional mutagenesis system, we used widely applicable transposons and distinguishable broad-range transformation markers, which should enable this system to be operational in nonmodel arthropods. In a pilot screen in Drosophila melanogaster, piggyBac mutator elements on the X chromosome were mobilized in males by a Hermes-based jumpstarter element providing piggyBac transposase activity under control of the alpha1-tubulin promoter. As primary reporters in the piggyBac mutator elements, we employed the heterologous transactivators GAL4delta or tTA. To identify larval and adult enhancer detectors, strains carrying UASp-EYFP or TRE-EYFP as secondary reporter elements were used. Tissue-specific enhancer activities were readily observed in the GAL4delta/UASp-based systems, but only rarely in the tTA/TRE system. Novel autosomal insertions were recovered with an average jumping rate of 80%. Of these novel insertions, 3.8% showed homozygous lethality, which was reversible by piggyBac excision. Insertions were found in both coding and noncoding regions of characterized genes and also in noncharacterized and non-P-targeted CG-number genes. This indicates that piggyBac will greatly facilitate the intended saturation mutagenesis in Drosophila. | lld:pubmed |
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| pubmed-article:12618403 | pubmed:language | eng | lld:pubmed |
| pubmed-article:12618403 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12618403 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:12618403 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12618403 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:12618403 | pubmed:month | Feb | lld:pubmed |
| pubmed-article:12618403 | pubmed:issn | 0016-6731 | lld:pubmed |
| pubmed-article:12618403 | pubmed:author | pubmed-author:HornCarstenC | lld:pubmed |
| pubmed-article:12618403 | pubmed:author | pubmed-author:WimmerErnst... | lld:pubmed |
| pubmed-article:12618403 | pubmed:author | pubmed-author:OffenNilsN | lld:pubmed |
| pubmed-article:12618403 | pubmed:author | pubmed-author:NystedtSverke... | lld:pubmed |
| pubmed-article:12618403 | pubmed:author | pubmed-author:HäckerUdoU | lld:pubmed |
| pubmed-article:12618403 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:12618403 | pubmed:volume | 163 | lld:pubmed |
| pubmed-article:12618403 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:12618403 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:12618403 | pubmed:pagination | 647-61 | lld:pubmed |
| pubmed-article:12618403 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
| pubmed-article:12618403 | pubmed:meshHeading | pubmed-meshheading:12618403... | lld:pubmed |
| pubmed-article:12618403 | pubmed:meshHeading | pubmed-meshheading:12618403... | lld:pubmed |
| pubmed-article:12618403 | pubmed:meshHeading | pubmed-meshheading:12618403... | lld:pubmed |
| pubmed-article:12618403 | pubmed:meshHeading | pubmed-meshheading:12618403... | lld:pubmed |
| pubmed-article:12618403 | pubmed:meshHeading | pubmed-meshheading:12618403... | lld:pubmed |
| pubmed-article:12618403 | pubmed:meshHeading | pubmed-meshheading:12618403... | lld:pubmed |
| pubmed-article:12618403 | pubmed:meshHeading | pubmed-meshheading:12618403... | lld:pubmed |
| pubmed-article:12618403 | pubmed:meshHeading | pubmed-meshheading:12618403... | lld:pubmed |
| pubmed-article:12618403 | pubmed:year | 2003 | lld:pubmed |
| pubmed-article:12618403 | pubmed:articleTitle | piggyBac-based insertional mutagenesis and enhancer detection as a tool for functional insect genomics. | lld:pubmed |
| pubmed-article:12618403 | pubmed:affiliation | Lehrstuhl für Genetik, Universität Bayreuth, 95447 Bayreuth, Germany. | lld:pubmed |
| pubmed-article:12618403 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:12618403 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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