Linked Life Data

12616690

Source:http://linkedlifedata.com/resource/pubmed/id/12616690

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2003-3-5
pubmed:abstractText
Using the carbon isotope labeling technique, the response of cyanobacterial central carbon metabolism to the change in environmental conditions was investigated. Synechocystis was grown in the heterotrophic and mixotrophic cultures fed with 13C-labeled glucose. The labeling patterns of the amino acids in biomass hydrolysates for both cultures were detected by the two-dimensional 1H-13C correlation nuclear magnetic resonance (2D 1H-13C COSY NMR) spectroscopy and gas chromatography-mass spectrometry (GC-MS) technique. The in vivo intracellular flux distributions were then quantitated from the labeling measurements and metabolite balances using a parameters fitting approach. From the estimated flux distributions, it was found that the pentose phosphate pathway was the major pathway of glucose catabolism in the heterotrophic culture, while in the mixotrophic culture, the flux of CO2 fixation through the Calvin cycle was about two-fold of the glucose input flux. The relative flux through the phosphoenolpyruvate carboxylase was very high in both cultures, and this reaction represented about 25% of the assimilated CO2 in the mixotrophic culture. More importantly, we found a substantial outflow from the tricarboxylic acid cycle to glycolysis pathway carried by the malic enzyme, demonstrating the operation of a C4 pathway in cyanobacterial cells through the PEP carboxylase and malic enzyme. The estimated flux distributions also revealed that the NADPH synthesis was in excess relative to its requirement, and the excess NADPH might be reoxidized in cyanobacterial respiration to provide the energy for cellular requirement. Moreover, the analyzed result also suggested that the activity of the respiratory electron transport chain in cyanobacterial cells was not inhibited by light.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
1096-7176
pubmed:author
pubmed:issnType
Print
pubmed:volume
4
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
202-16
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:12616690-Amino Acids, pubmed-meshheading:12616690-Carbon, pubmed-meshheading:12616690-Carbon Radioisotopes, pubmed-meshheading:12616690-Cells, Cultured, pubmed-meshheading:12616690-Computer Simulation, pubmed-meshheading:12616690-Culture Media, pubmed-meshheading:12616690-Cyanobacteria, pubmed-meshheading:12616690-Gas Chromatography-Mass Spectrometry, pubmed-meshheading:12616690-Glucose, pubmed-meshheading:12616690-Hydrolysis, pubmed-meshheading:12616690-Isotope Labeling, pubmed-meshheading:12616690-Models, Biological, pubmed-meshheading:12616690-Models, Chemical, pubmed-meshheading:12616690-Multienzyme Complexes, pubmed-meshheading:12616690-Nuclear Magnetic Resonance, Biomolecular, pubmed-meshheading:12616690-Species Specificity, pubmed-meshheading:12616690-Stereoisomerism
pubmed:year
2002
pubmed:articleTitle
Metabolic flux analysis in Synechocystis using isotope distribution from 13C-labeled glucose.
pubmed:affiliation
Department of Biochemical Engineering & Science, Kyushu Institute of Technology, Iizuka, Fukuoka 820-8502, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't