Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2003-2-28
pubmed:abstractText
To gain a better understanding of the critical role of mitochondria in cell function, we have compiled an extensive catalogue of the mitochondrial proteome using highly purified mitochondria from normal human heart tissue. Sucrose gradient centrifugation was employed to partially resolve protein complexes whose individual protein components were separated by one-dimensional PAGE. Total in-gel processing and subsequent detection by mass spectrometry and rigorous bioinformatic analysis yielded a total of 615 distinct protein identifications. All protein pI values, molecular weight ranges, and hydrophobicities were represented. The coverage of the known subunits of the oxidative phosphorylation machinery within the inner mitochondrial membrane was >90%. A significant proportion of identified proteins are involved in signaling, RNA, DNA, and protein synthesis, ion transport, and lipid metabolism. The biochemical roles of 19% of the identified proteins have not been defined. This database of proteins provides a comprehensive resource for the discovery of novel mitochondrial functions and pathways.
pubmed:commentsCorrections
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
1087-0156
pubmed:author
pubmed:issnType
Print
pubmed:volume
21
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
281-6
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:12592411-Adolescent, pubmed-meshheading:12592411-Adult, pubmed-meshheading:12592411-Amino Acid Sequence, pubmed-meshheading:12592411-Animals, pubmed-meshheading:12592411-Cells, Cultured, pubmed-meshheading:12592411-Databases, Protein, pubmed-meshheading:12592411-Electrophoresis, pubmed-meshheading:12592411-Heart, pubmed-meshheading:12592411-Humans, pubmed-meshheading:12592411-Information Storage and Retrieval, pubmed-meshheading:12592411-Mass Spectrometry, pubmed-meshheading:12592411-Middle Aged, pubmed-meshheading:12592411-Mitochondria, pubmed-meshheading:12592411-Mitochondrial Proteins, pubmed-meshheading:12592411-Molecular Weight, pubmed-meshheading:12592411-Myocardium, pubmed-meshheading:12592411-Proteome, pubmed-meshheading:12592411-Proteomics, pubmed-meshheading:12592411-Sequence Analysis, Protein
pubmed:year
2003
pubmed:articleTitle
Characterization of the human heart mitochondrial proteome.
pubmed:affiliation
MitoKor, 11494 Sorrento Valley Road, San Diego, California 92121, USA. taylors@mitokor.com
pubmed:publicationType
Journal Article