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rdf:type |
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lifeskim:mentions |
umls-concept:C0017262,
umls-concept:C0040845,
umls-concept:C0185117,
umls-concept:C0220839,
umls-concept:C0243144,
umls-concept:C0392756,
umls-concept:C0442805,
umls-concept:C0667093,
umls-concept:C1538931,
umls-concept:C1854053,
umls-concept:C2911684
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pubmed:issue |
4
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pubmed:dateCreated |
2003-2-3
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pubmed:abstractText |
The Na+-dependent glutamate transporter EAAT3 facilitates glutamate uptake into neurons as well as many other cell types. GTRAP3-18 (JWA, Arl6ip5) is a novel protein that interacts with EAAT3 and negatively modulates EAAT3-mediated glutamate uptake. Previous studies suggest that retinoic acid (RA) decreases Na+-dependent glutamate uptake and increases GTRAP3-18 protein expression. However, the RA used in those studies was complexed with methyl-beta-cyclodextrin (MebetaCD). In the present study we found that MebetaCD, but not RA, significantly reduced Na+-dependent EAAT3-mediated [3H]glutamate uptake in human embryonic kidney 293 (HEK293) cells. MebetaCD also significantly increased GTRAP3-18 protein expression in HEK293 cells as well as in rat hypothalamic neuron cultures. Intracerebroventricular administration of MebetaCD to the mouse brain resulted in a significant increase in GTRAP3-18 immunoreactivity in the hippocampus and cerebral cortex. In conclusion, we have shown that MebetaCD reduces EAAT3-mediated glutamate uptake and induces the expression of GTRAP3-18 protein.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Amino Acid Transport System X-AG,
http://linkedlifedata.com/resource/pubmed/chemical/Arl6ip5 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Carrier Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclodextrins,
http://linkedlifedata.com/resource/pubmed/chemical/Excitatory Amino Acid Transporter 3,
http://linkedlifedata.com/resource/pubmed/chemical/Glutamate Plasma Membrane...,
http://linkedlifedata.com/resource/pubmed/chemical/Glutamic Acid,
http://linkedlifedata.com/resource/pubmed/chemical/SLC1A1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Slc1a1 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Sodium,
http://linkedlifedata.com/resource/pubmed/chemical/Symporters,
http://linkedlifedata.com/resource/pubmed/chemical/Tretinoin,
http://linkedlifedata.com/resource/pubmed/chemical/beta-Cyclodextrins,
http://linkedlifedata.com/resource/pubmed/chemical/methyl-beta-cyclodextrin
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0022-3042
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
84
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
891-4
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:12562531-Amino Acid Transport System X-AG,
pubmed-meshheading:12562531-Animals,
pubmed-meshheading:12562531-Carrier Proteins,
pubmed-meshheading:12562531-Cell Line,
pubmed-meshheading:12562531-Cells, Cultured,
pubmed-meshheading:12562531-Cerebral Cortex,
pubmed-meshheading:12562531-Cyclodextrins,
pubmed-meshheading:12562531-Excitatory Amino Acid Transporter 3,
pubmed-meshheading:12562531-Glutamate Plasma Membrane Transport Proteins,
pubmed-meshheading:12562531-Glutamic Acid,
pubmed-meshheading:12562531-Hippocampus,
pubmed-meshheading:12562531-Humans,
pubmed-meshheading:12562531-Hypothalamus,
pubmed-meshheading:12562531-Injections, Intraventricular,
pubmed-meshheading:12562531-Kidney,
pubmed-meshheading:12562531-Male,
pubmed-meshheading:12562531-Mice,
pubmed-meshheading:12562531-Mice, Inbred C57BL,
pubmed-meshheading:12562531-Neurons,
pubmed-meshheading:12562531-Sodium,
pubmed-meshheading:12562531-Symporters,
pubmed-meshheading:12562531-Tretinoin,
pubmed-meshheading:12562531-beta-Cyclodextrins
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pubmed:year |
2003
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pubmed:articleTitle |
Methyl-beta-cyclodextrin but not retinoic acid reduces EAAT3-mediated glutamate uptake and increases GTRAP3-18 expression.
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pubmed:affiliation |
Department of Neuroscience, Ohio State Biochemistry Program, The Ohio State University, 333 West 10th Avenue, Columbus, OH 43210-1239, USA.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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