Linked Life Data

12560564

Source:http://linkedlifedata.com/resource/pubmed/id/12560564

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
Pt 2
pubmed:dateCreated
2003-1-31
pubmed:abstractText
Kaposi's sarcoma-associated herpesvirus (KSHV)/human herpesvirus-8-encoded viral interferon regulatory factor (vIRF) transforms NIH3T3 cells, represses interferon signal transduction and regulates the expression of other KSHV genes. Here, we have shown that vIRF is a transcriptional activator and auto-activates its own expression. Ectopic expression of vIRF activated the vIRF promoter in KSHV-negative 293, COS7, HeLa and BJAB cell lines in a dose-dependent fashion in a reporter assay and the expression of vIRF transcripts from endogenous viral genomes in BCBL-1 and BC-1 cells latently infected with KSHV. Deletion analysis identified two cis elements, named Vac1 and Vac2, in the vIRF promoter that were responsive to vIRF activation. vIRF auto-activation via Vac1 but not Vac2 was repressed by Tis, a transcriptional silencer in the vIRF promoter. Neither Vac1 nor Vac2 contain any interferon-stimulated response element (ISRE)-like sequences and are unresponsive to induction with interferon-beta and -gamma. These results indicate that KSHV uses the mechanism of auto-activation to regulate the expression of a viral transforming protein to efficiently evade host tumour suppressor pathways.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0022-1317
pubmed:author
pubmed:issnType
Print
pubmed:volume
84
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
329-36
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:12560564-Animals, pubmed-meshheading:12560564-COS Cells, pubmed-meshheading:12560564-Cell Line, pubmed-meshheading:12560564-Cell Line, Transformed, pubmed-meshheading:12560564-Cell Transformation, Viral, pubmed-meshheading:12560564-DNA-Binding Proteins, pubmed-meshheading:12560564-Enhancer Elements, Genetic, pubmed-meshheading:12560564-Gene Expression Regulation, Viral, pubmed-meshheading:12560564-HeLa Cells, pubmed-meshheading:12560564-Herpesvirus 8, Human, pubmed-meshheading:12560564-Humans, pubmed-meshheading:12560564-Interferon Regulatory Factors, pubmed-meshheading:12560564-Promoter Regions, Genetic, pubmed-meshheading:12560564-Transcription, Genetic, pubmed-meshheading:12560564-Transcription Factors, pubmed-meshheading:12560564-Transcriptional Activation, pubmed-meshheading:12560564-Viral Proteins
pubmed:year
2003
pubmed:articleTitle
Auto-activation of the transforming viral interferon regulatory factor encoded by Kaposi's sarcoma-associated herpesvirus (human herpesvirus-8).
pubmed:affiliation
Tumor Virology Program, Children's Cancer Research Institute, The University of Texas Health Science Center at San Antonio, 7703 Floyd Curl Drive, 78229-3900, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't