Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2003-1-28
pubmed:abstractText
Seeds of sainfoin (Onobrychis vicaefolia Scop.) were carried in the recoverable satellite 940703 and recovered from earth orbit from China in 1994. The progeny seeds were obtained by producing in field. The salt tolerant calluses were selected by screening seedling and callus on 1.5% NaCl-containing medium, reviving growth on NaCl- free medium and selecting callus on 1.2% NaCl-containing medium. The salt tolerant line callus maintained the normal ability to regenerate plant. The salt tolerant line callus exhibited cross-resistance to PEG stress. The variant appeared higher efficiency than control to accumulate proline under salt stress, however, under nonstress condition it had lower proline level than control, which suggested that the higher efficiency to synthesize proline under stress condition may be more important than higher level in tissue under nonstress condition. The mechanism of proline synthesis in the selected callus was considered to result from the alteration in gene sensitivity to water regulation at transcription level. Acrylamide gradient electrophoresis showed that new isoenzyme form with MW 175 kD and 75 kD of superoxide dismutase and esterase respectively appeared in salt torlerant callus. It was indicated that the combination of space mutagenesis with tissue culture could be used for the selection of salt tolerant sainfoin line in vitro.
pubmed:language
chi
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0001-5334
pubmed:author
pubmed:issnType
Print
pubmed:volume
34
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
11-5
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed:year
2001
pubmed:articleTitle
[Selection and identification of salt tolerant line of sainfoin from the seeds of first post-flight plants].
pubmed:affiliation
Institute of Botany, Chinese Academy of Sciences, Beijing 100093.
pubmed:publicationType
Journal Article, English Abstract, Research Support, Non-U.S. Gov't