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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
2003-1-21
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pubmed:abstractText |
Protein kinase B (PKB), a kinase downstream of phosphatidylinositol 3-kinase (PI3-kinase) provides anti-apoptotic and survival signals via phosphorylation of various targets. Inhibiting PI3-kinase with a 12 h exposure to 10 microM LY294002 induces levels of apoptosis of 30.39+/-1.53% in the KB-V1 multidrug resistant (MDR) cell line compared to 4.54+/-1.00% in drug sensitive KB-3-1 cells (P<0.001). This occurred in conjunction with a preferential reduction in activated PKB in MDR cells. These results suggest the PI3-kinase/PKB signalling pathway is important for the survival of MDR cells and inhibition of this pathway results in the selective induction of apoptosis in MDR cells.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/2-(4-morpholinyl)-8-phenyl-4H-1-benz...,
http://linkedlifedata.com/resource/pubmed/chemical/Chromones,
http://linkedlifedata.com/resource/pubmed/chemical/Culture Media, Serum-Free,
http://linkedlifedata.com/resource/pubmed/chemical/Enzyme Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/Morpholines,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphatidylinositol 3-Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Poly(ADP-ribose) Polymerases,
http://linkedlifedata.com/resource/pubmed/chemical/Protein-Serine-Threonine Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins c-akt,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0304-3835
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
10
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pubmed:volume |
190
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
31-6
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pubmed:dateRevised |
2010-11-18
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pubmed:meshHeading |
pubmed-meshheading:12536074-Apoptosis,
pubmed-meshheading:12536074-Blotting, Western,
pubmed-meshheading:12536074-Cell Line,
pubmed-meshheading:12536074-Cell Survival,
pubmed-meshheading:12536074-Chromones,
pubmed-meshheading:12536074-Culture Media, Serum-Free,
pubmed-meshheading:12536074-Drug Resistance, Neoplasm,
pubmed-meshheading:12536074-Enzyme Inhibitors,
pubmed-meshheading:12536074-Humans,
pubmed-meshheading:12536074-Morpholines,
pubmed-meshheading:12536074-Neoplasms,
pubmed-meshheading:12536074-Phenotype,
pubmed-meshheading:12536074-Phosphatidylinositol 3-Kinases,
pubmed-meshheading:12536074-Poly(ADP-ribose) Polymerases,
pubmed-meshheading:12536074-Protein-Serine-Threonine Kinases,
pubmed-meshheading:12536074-Proto-Oncogene Proteins,
pubmed-meshheading:12536074-Proto-Oncogene Proteins c-akt,
pubmed-meshheading:12536074-RNA, Messenger,
pubmed-meshheading:12536074-Signal Transduction,
pubmed-meshheading:12536074-Time Factors,
pubmed-meshheading:12536074-Tumor Cells, Cultured
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pubmed:year |
2003
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pubmed:articleTitle |
LY294002, an inhibitor of phosphatidylinositol-3-kinase, causes preferential induction of apoptosis in human multidrug resistant cells.
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pubmed:affiliation |
Department of Biology, University of York, PO Box 373, York YO10 5YW, UK.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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