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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
2003-1-21
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pubmed:abstractText |
Opioid receptors in the ventral tegmental area, predominantly the mu-opioid receptors, have been suggested to modulate reinforcement sensitivity for both opioid and non-opioid drugs of abuse. The present study was conducted to study signal transduction proteins, which may mediate the functioning of mu-opioid receptors in the neurons of the ventral tegmental area. Therefore, brain slices of the ventral tegmental area were exposed in vitro to the specific mu-opioid agonist fentanyl and immunohistochemically stained for four different activated proteins using phospho-specific antibodies. Fentanyl dose-dependently activated extracellular signal-regulated protein in brain slices of the ventral tegmental area. This activation was reversible with naloxone. Furthermore, naloxone itself also activated extracellular signal-regulated protein kinase. Under the present conditions fentanyl did not affect extracellular signal-regulated protein kinase 1 and 2, Stat and cyclic AMP-response element-binding protein activity. The direct activation of extracellular signal-regulated protein kinase in ventral tegmental area slices by the mu-opioid agonist fentanyl may suggest a role of extracellular signal-regulated protein kinase in reward processes.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Analgesics, Opioid,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclic AMP Response...,
http://linkedlifedata.com/resource/pubmed/chemical/Fentanyl,
http://linkedlifedata.com/resource/pubmed/chemical/MAP Kinase Kinase 1,
http://linkedlifedata.com/resource/pubmed/chemical/MAP Kinase Kinase 2,
http://linkedlifedata.com/resource/pubmed/chemical/Mitogen-Activated Protein Kinase...,
http://linkedlifedata.com/resource/pubmed/chemical/Mitogen-Activated Protein Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Protein-Serine-Threonine Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Protein-Tyrosine Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Opioid, mu
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pubmed:status |
MEDLINE
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pubmed:issn |
0306-4522
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pubmed:author |
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pubmed:copyrightInfo |
Copyright 2003 IBRO
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pubmed:issnType |
Print
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pubmed:volume |
116
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
139-44
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:12535947-Analgesics, Opioid,
pubmed-meshheading:12535947-Animals,
pubmed-meshheading:12535947-Blotting, Western,
pubmed-meshheading:12535947-Cyclic AMP Response Element-Binding Protein,
pubmed-meshheading:12535947-Enzyme Activation,
pubmed-meshheading:12535947-Fentanyl,
pubmed-meshheading:12535947-Immunohistochemistry,
pubmed-meshheading:12535947-MAP Kinase Kinase 1,
pubmed-meshheading:12535947-MAP Kinase Kinase 2,
pubmed-meshheading:12535947-Male,
pubmed-meshheading:12535947-Mitogen-Activated Protein Kinase Kinases,
pubmed-meshheading:12535947-Mitogen-Activated Protein Kinases,
pubmed-meshheading:12535947-Phosphorylation,
pubmed-meshheading:12535947-Protein-Serine-Threonine Kinases,
pubmed-meshheading:12535947-Protein-Tyrosine Kinases,
pubmed-meshheading:12535947-Rats,
pubmed-meshheading:12535947-Rats, Wistar,
pubmed-meshheading:12535947-Receptors, Opioid, mu,
pubmed-meshheading:12535947-Signal Transduction,
pubmed-meshheading:12535947-Ventral Tegmental Area
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pubmed:year |
2003
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pubmed:articleTitle |
ERK1/2 activation in rat ventral tegmental area by the mu-opioid agonist fentanyl: an in vitro study.
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pubmed:affiliation |
Division of Pharmacology and Anatomy, Rudolf Magnus Institute for Neurosciences, University Medical Center, Universiteitsweg 100, 3584 CG Utrecht, The Netherlands.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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