Source:http://linkedlifedata.com/resource/pubmed/id/12529556
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
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| pubmed:dateCreated |
2003-1-16
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| pubmed:abstractText |
Hematopoietic and hepatic stem cells share characteristic markers such as CD34, c-kit, and Thy1. Based on the recent observations that hepatocytes may originate from bone marrow, we investigated the potential of CD34(+) bone marrow cells to differentiate into hepatocytic cells in vitro. CD34(+) and CD34(-) human bone marrow cells were separated by magnetic cell sorting. Cells were cultured on a collagen matrix in a defined medium containing hepatocyte growth factor. Cell count and size were measured by flow cytometry, and reverse transcription polymerase chain reaction was carried out for the liver-specific markers CK-19 and albumin. During cell culture, CD34(+) cells showed an increasing cell number and proliferative activity as assessed by Ki-67 staining. Under the specified culture conditions, CD34(+) cells expressed albumin RNA and CK-19 RNA after 28 days, whereas CD34(-) cells did not show liver-specific gene expression. The results indicate that CD34(+) adult human bone marrow stem cells can differentiate into hepatocytic cells in vitro.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical | |
| pubmed:status |
MEDLINE
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| pubmed:issn |
1066-5099
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:volume |
21
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
98-104
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| pubmed:dateRevised |
2004-11-17
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| pubmed:meshHeading |
pubmed-meshheading:12529556-Adult,
pubmed-meshheading:12529556-Albumins,
pubmed-meshheading:12529556-Antigens, CD34,
pubmed-meshheading:12529556-Bone Marrow,
pubmed-meshheading:12529556-Bone Marrow Transplantation,
pubmed-meshheading:12529556-Cell Count,
pubmed-meshheading:12529556-Cell Differentiation,
pubmed-meshheading:12529556-Cells, Cultured,
pubmed-meshheading:12529556-Female,
pubmed-meshheading:12529556-Flow Cytometry,
pubmed-meshheading:12529556-Gene Expression Regulation,
pubmed-meshheading:12529556-Hematopoietic Stem Cells,
pubmed-meshheading:12529556-Hepatocytes,
pubmed-meshheading:12529556-Humans,
pubmed-meshheading:12529556-Immunomagnetic Separation,
pubmed-meshheading:12529556-Ki-67 Antigen,
pubmed-meshheading:12529556-Liver,
pubmed-meshheading:12529556-Male,
pubmed-meshheading:12529556-Organ Specificity,
pubmed-meshheading:12529556-RNA, Messenger,
pubmed-meshheading:12529556-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:12529556-Stem Cells,
pubmed-meshheading:12529556-Transplantation, Homologous
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| pubmed:year |
2003
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| pubmed:articleTitle |
Liver-specific gene expression in cultured human hematopoietic stem cells.
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| pubmed:affiliation |
Bone Marrow Transplantation Center, Universitätsklinikum Hamburg-Eppendorf, Hamburg, Germany. fiegel@uke.uni-hamburg.de
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| pubmed:publicationType |
Journal Article
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