Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
2002-12-30
pubmed:databankReference
pubmed:abstractText
Human 11p15.5, as well as its orthologous mouse 7F4/F5, is known as the imprinting domain extending from IPL/Ipl to H19. OBPH1 and Obph1 are located beyond the presumed imprinting boundary on the IPL/Ipl side. We determined full-length cDNAs and complete genomic structures of both orthologues. We also investigated their precise imprinting and methylation status. The orthologues resembled each other in genomic structure and in the position of the 5' CpG island and were expressed ubiquitously. OBPH1 and Obph1 were predominantly expressed from the maternal allele only in placenta, with hypo- and not differentially methylated 5' CpG islands in both species. These results suggested that the imprinting domain would extend beyond the presumed imprinting boundary and that methylation of the 5' CpG island was not associated with the imprinting status in either species. It remains to be elucidated whether the gene is under the control of the KIP2/LIT1 subdomain or is regulated by a specific mechanism. Analysis of the precise genomic sequence around the region should help resolve this question.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0888-7543
pubmed:author
pubmed:issnType
Print
pubmed:volume
80
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
575-84
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:12504849-Alleles, pubmed-meshheading:12504849-Amino Acid Sequence, pubmed-meshheading:12504849-Animals, pubmed-meshheading:12504849-Base Sequence, pubmed-meshheading:12504849-Chromosome Mapping, pubmed-meshheading:12504849-Chromosomes, Human, Pair 11, pubmed-meshheading:12504849-CpG Islands, pubmed-meshheading:12504849-DNA, pubmed-meshheading:12504849-DNA Methylation, pubmed-meshheading:12504849-Female, pubmed-meshheading:12504849-Gene Expression, pubmed-meshheading:12504849-Genomic Imprinting, pubmed-meshheading:12504849-Humans, pubmed-meshheading:12504849-Mice, pubmed-meshheading:12504849-Mice, Inbred C57BL, pubmed-meshheading:12504849-Molecular Sequence Data, pubmed-meshheading:12504849-Polymorphism, Single Nucleotide, pubmed-meshheading:12504849-Receptors, Steroid, pubmed-meshheading:12504849-Sequence Analysis, DNA, pubmed-meshheading:12504849-Sequence Homology, Amino Acid, pubmed-meshheading:12504849-Synteny
pubmed:year
2002
pubmed:articleTitle
Characterization and imprinting status of OBPH1/Obph1 gene: implications for an extended imprinting domain in human and mouse.
pubmed:affiliation
Department of Biomolecular Sciences, Saga Medical School, 5-1-1 Nabeshima, Saga, 849-8501, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't