A UV/UV system for the spectrophotometric determination of sugars during wine production is described. The methodology is based on the formation of a UV-absorbing byproduct produced by the photodegradation of sugars. Under the given experimental conditions, the influence of increasing the amount of acids and alcohol is negligible. The measurement by UV/UV is compared with FTIR as reference method and validated according to the French norm AFNOR XPT 90 210.