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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
9
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pubmed:dateCreated |
2002-12-23
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pubmed:abstractText |
As antioxidants, polyphenols are considered to be potentially useful in preventing chronic diseases in man, including Parkinson's disease (PD), a disease involving dopamine (DA) neurons. Our studies have demonstrated that polyphenols extracted from green tea (GT) can inhibit the uptake of 3H-dopamine (3H-DA) and 1-methyl-4-phenylpyridinium (MPP(+)) by DA transporters (DAT) and partially protect embryonic rat mesencephalic dopaminergic (DAergic) neurons from MPP(+)-induced injury. The inhibitory effects of GT polyphenols on 3H-DA uptake were determined in DAT-pCDNA3-transfected Chinese Hamster Ovary (DAT-CHO) cells and in striatal synaptosomes of C57BL/6 mice in vitro and in vivo. The inhibitory effects on 3H-MPP(+) uptake were determined in primary cultures of embryonic rat mesencephalic DAergic cells. Inhibition of uptake for both 3H-DA and 3H-MPP(+) was dose-dependent in the presence of polyphenols. Incubation with 50 microM MPP(+) resulted in a significant loss of tyrosine-hydroxylase (TH)-positive cells in the primary embryonic mesencephalic cultures, while pretreatment with polyphenols (10 to 30 microg/ml) or mazindol (10 microM), a classical DAT inhibitor, significantly attenuated MPP(+)-induced loss of TH-positive cells. These results suggest that GT polyphenols have inhibitory effects on DAT, through which they block MPP(+) uptake and protect DAergic neurons against MPP(+)-induced injury.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/1-Methyl-4-phenylpyridinium,
http://linkedlifedata.com/resource/pubmed/chemical/Dopamine Uptake Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/Flavonoids,
http://linkedlifedata.com/resource/pubmed/chemical/Mazindol,
http://linkedlifedata.com/resource/pubmed/chemical/Neuroprotective Agents,
http://linkedlifedata.com/resource/pubmed/chemical/Phenols,
http://linkedlifedata.com/resource/pubmed/chemical/Plant Extracts,
http://linkedlifedata.com/resource/pubmed/chemical/Polymers,
http://linkedlifedata.com/resource/pubmed/chemical/Polyphenols,
http://linkedlifedata.com/resource/pubmed/chemical/Tea,
http://linkedlifedata.com/resource/pubmed/chemical/Tyrosine 3-Monooxygenase
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0024-3205
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
17
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pubmed:volume |
72
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1073-83
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
pubmed-meshheading:12495785-1-Methyl-4-phenylpyridinium,
pubmed-meshheading:12495785-Animals,
pubmed-meshheading:12495785-CHO Cells,
pubmed-meshheading:12495785-Cricetinae,
pubmed-meshheading:12495785-Dopamine,
pubmed-meshheading:12495785-Dopamine Uptake Inhibitors,
pubmed-meshheading:12495785-Dose-Response Relationship, Drug,
pubmed-meshheading:12495785-Flavonoids,
pubmed-meshheading:12495785-Male,
pubmed-meshheading:12495785-Mazindol,
pubmed-meshheading:12495785-Mice,
pubmed-meshheading:12495785-Mice, Inbred C57BL,
pubmed-meshheading:12495785-Neurons,
pubmed-meshheading:12495785-Neuroprotective Agents,
pubmed-meshheading:12495785-Phenols,
pubmed-meshheading:12495785-Plant Extracts,
pubmed-meshheading:12495785-Polymers,
pubmed-meshheading:12495785-Polyphenols,
pubmed-meshheading:12495785-Rats,
pubmed-meshheading:12495785-Rats, Sprague-Dawley,
pubmed-meshheading:12495785-Synaptosomes,
pubmed-meshheading:12495785-Tea,
pubmed-meshheading:12495785-Transfection,
pubmed-meshheading:12495785-Tyrosine 3-Monooxygenase
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pubmed:year |
2003
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pubmed:articleTitle |
Effects of green tea polyphenols on dopamine uptake and on MPP+ -induced dopamine neuron injury.
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pubmed:affiliation |
Department of Neurology, Baylor College of Medicine, One Baylor Plaza, Houston, TX 77030, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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