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rdf:type |
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lifeskim:mentions |
umls-concept:C0013030,
umls-concept:C0030685,
umls-concept:C0033634,
umls-concept:C0391871,
umls-concept:C0597357,
umls-concept:C0680255,
umls-concept:C0851285,
umls-concept:C0851827,
umls-concept:C1283071,
umls-concept:C1701901,
umls-concept:C1963578
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pubmed:issue |
1
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pubmed:dateCreated |
2002-12-19
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pubmed:abstractText |
We have previously shown that sigma1 receptor agonists inhibit N-methyl-D-aspartate (NMDA)-stimulated [3H]dopamine from slices of rat striatum in a concentration-related manner and that the inhibition is reversed by sigma1 receptor-selective and nonsubtype-selective sigma receptor antagonists. Based on previous evidence from our laboratory as well as other laboratories, we hypothesized that sigma1 receptors might use a protein kinase C (PKC) signaling pathway to modulate stimulated dopamine release. We tested several inhibitors of PKC isozymes, as well as a phospholipase C inhibitor for their effects on sigma1 receptor agonist-mediated regulation of [3H]dopamine release. Although none of the inhibitors tested affected the ability of NMDA to stimulate [3H]dopamine release, they all abolished regulation by the sigma1 receptor agonist (+)-pentazocine in a concentration-related manner. We also found that prior exposure to 1 microM phorbol 2-myristate 13-acetate for 30 min abolished regulation by (+)-pentazocine. We concluded that an intact PKC system was required for sigma1 agonist-mediated regulation of NMDA-stimulated [3H]dopamine release from rat striatal slices. Based on the pharmacological profile of the PKC inhibitors tested, as well as reports in the literature on PKC
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Diglycerides,
http://linkedlifedata.com/resource/pubmed/chemical/Enzyme Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/Excitatory Amino Acid Agonists,
http://linkedlifedata.com/resource/pubmed/chemical/Isoenzymes,
http://linkedlifedata.com/resource/pubmed/chemical/N-Methylaspartate,
http://linkedlifedata.com/resource/pubmed/chemical/Narcotic Antagonists,
http://linkedlifedata.com/resource/pubmed/chemical/Pentazocine,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Kinase C,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, sigma,
http://linkedlifedata.com/resource/pubmed/chemical/Tetradecanoylphorbol Acetate,
http://linkedlifedata.com/resource/pubmed/chemical/Type C Phospholipases,
http://linkedlifedata.com/resource/pubmed/chemical/sigma-1 receptor
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0022-3565
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
304
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
364-9
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:12490613-Animals,
pubmed-meshheading:12490613-Diglycerides,
pubmed-meshheading:12490613-Dopamine,
pubmed-meshheading:12490613-Dose-Response Relationship, Drug,
pubmed-meshheading:12490613-Enzyme Inhibitors,
pubmed-meshheading:12490613-Excitatory Amino Acid Agonists,
pubmed-meshheading:12490613-Isoenzymes,
pubmed-meshheading:12490613-Male,
pubmed-meshheading:12490613-N-Methylaspartate,
pubmed-meshheading:12490613-Narcotic Antagonists,
pubmed-meshheading:12490613-Neostriatum,
pubmed-meshheading:12490613-Pentazocine,
pubmed-meshheading:12490613-Protein Kinase C,
pubmed-meshheading:12490613-Rats,
pubmed-meshheading:12490613-Rats, Sprague-Dawley,
pubmed-meshheading:12490613-Receptors, sigma,
pubmed-meshheading:12490613-Tetradecanoylphorbol Acetate,
pubmed-meshheading:12490613-Type C Phospholipases
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pubmed:year |
2003
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pubmed:articleTitle |
Sigma1 receptor agonist-mediated regulation of N-methyl-D-aspartate-stimulated [3H]dopamine release is dependent upon protein kinase C.
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pubmed:affiliation |
Department of Pharmacology, The George Washington University Medical Center, Washington, DC 20037, USA.
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pubmed:publicationType |
Journal Article,
In Vitro,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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