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rdf:type |
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lifeskim:mentions |
umls-concept:C0007589,
umls-concept:C0014257,
umls-concept:C0086035,
umls-concept:C0229601,
umls-concept:C0332152,
umls-concept:C0378781,
umls-concept:C0443196,
umls-concept:C0796355,
umls-concept:C1416745,
umls-concept:C1425354,
umls-concept:C1511938,
umls-concept:C1522240,
umls-concept:C1707391,
umls-concept:C1881379
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pubmed:issue |
24
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pubmed:dateCreated |
2002-12-17
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pubmed:abstractText |
Hematopoiesis in most vertebrate species occurs in two distinct phases, primitive and definitive, which diverge from FLK1(+)VE-cadherin(-) mesoderm and FLK1(+)VE-cadherin(+) endothelial cells (EC), respectively. This study aimed at determining the stage at which hematopoietic lineage fate is determined by manipulating the SCL/tal-1 expression that is known to be essential for the early development of the primitive and definitive hematopoietic systems. We established SCL-null ES cell lines in which SCL expression is rescued by tamoxifen-inducible Cre recombinase-loxP site-mediated recombination. While no hematopoietic cells (HPC) were detected in SCL-null ES cell differentiation cultures, SCL gene reactivation from day 2 to day 4 after initiation of differentiation could rescue both primitive and definitive hematopoiesis. SCL reactivation at later phases was ineffective. Moreover, generation of VE-cadherin(+) EC that can give rise to definitive HPC required SCL reactivation prior to VE-cadherin expression. These results indicated that the competence to become HPC is acquired at the mesodermal stage by a SCL-dependent process that takes place independently of determination of endothelial fate.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/12485991-10618416,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12485991-10652346,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12485991-10661871,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12485991-10804185,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12485991-11226262,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12485991-11458531,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12485991-11493549,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12485991-11737271,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12485991-12049718,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12485991-12049719,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12485991-12091336,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12485991-1370462,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12485991-7624372,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12485991-7830794,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12485991-7889417,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12485991-8066449,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12485991-8118024,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12485991-8414504,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12485991-8808625,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12485991-8990620,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12485991-9087406,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12485991-9169850,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12485991-9269761,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12485991-9472016,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12485991-9499398,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12485991-9521912,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12485991-9636076,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12485991-9655490,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12485991-9670018,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12485991-9778515,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12485991-9949168
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0261-4189
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
16
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pubmed:volume |
21
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
6700-8
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:12485991-Animals,
pubmed-meshheading:12485991-Basic Helix-Loop-Helix Transcription Factors,
pubmed-meshheading:12485991-Blotting, Northern,
pubmed-meshheading:12485991-Cell Differentiation,
pubmed-meshheading:12485991-Cell Line,
pubmed-meshheading:12485991-Cell Lineage,
pubmed-meshheading:12485991-Cell Separation,
pubmed-meshheading:12485991-Chickens,
pubmed-meshheading:12485991-DNA-Binding Proteins,
pubmed-meshheading:12485991-Electroporation,
pubmed-meshheading:12485991-Embryo, Mammalian,
pubmed-meshheading:12485991-Embryo, Nonmammalian,
pubmed-meshheading:12485991-Endothelium,
pubmed-meshheading:12485991-Flow Cytometry,
pubmed-meshheading:12485991-Hematopoiesis,
pubmed-meshheading:12485991-Hematopoietic Stem Cells,
pubmed-meshheading:12485991-Kinetics,
pubmed-meshheading:12485991-Mesoderm,
pubmed-meshheading:12485991-Mice,
pubmed-meshheading:12485991-Models, Biological,
pubmed-meshheading:12485991-Mutation,
pubmed-meshheading:12485991-Plasmids,
pubmed-meshheading:12485991-Protein Structure, Tertiary,
pubmed-meshheading:12485991-Proto-Oncogene Proteins,
pubmed-meshheading:12485991-Time Factors,
pubmed-meshheading:12485991-Transcription Factors
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pubmed:year |
2002
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pubmed:articleTitle |
SCL/tal-1-dependent process determines a competence to select the definitive hematopoietic lineage prior to endothelial differentiation.
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pubmed:affiliation |
Department of Molecular Genetics, Graduate School of Medicine, Kyoto University, Kyoto, 606-8057, Japan. mendoh@virus.kyoto-u.ac.jp
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