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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
2002-12-16
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pubmed:abstractText |
The cap-binding protein eIF4E-binding protein 3 (4E-BP3) was identified some years ago, but its properties have not been investigated in detail. In this report, we investigated the regulation and localisation of 4E-BP3. We show that 4E-BP3 is present in the nucleus as well as in the cytoplasm in primary T cells, HEK293 cells and HeLa cells. 4E-BP3 was associated with eIF4E in both cell compartments. Furthermore, 4E-BP3/eIF4E association in the cytoplasm was regulated by serum or interleukin-2 starvation in the different cell types. Rapamycin did not affect the association of eIF4E with 4E-BP3 in the cytoplasm or in the nucleus.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/7-methylguanosine triphosphate,
http://linkedlifedata.com/resource/pubmed/chemical/Carrier Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Culture Media, Serum-Free,
http://linkedlifedata.com/resource/pubmed/chemical/EIF4EBP3 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Eukaryotic Initiation Factor-4E,
http://linkedlifedata.com/resource/pubmed/chemical/Immunosuppressive Agents,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-2,
http://linkedlifedata.com/resource/pubmed/chemical/Peptides,
http://linkedlifedata.com/resource/pubmed/chemical/RNA Cap Analogs,
http://linkedlifedata.com/resource/pubmed/chemical/Sirolimus
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0014-5793
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
18
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pubmed:volume |
532
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
319-23
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:12482586-Blotting, Western,
pubmed-meshheading:12482586-Carrier Proteins,
pubmed-meshheading:12482586-Cell Line,
pubmed-meshheading:12482586-Cell Nucleus,
pubmed-meshheading:12482586-Culture Media, Serum-Free,
pubmed-meshheading:12482586-Cytoplasm,
pubmed-meshheading:12482586-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:12482586-Eukaryotic Initiation Factor-4E,
pubmed-meshheading:12482586-Gene Expression Regulation,
pubmed-meshheading:12482586-HeLa Cells,
pubmed-meshheading:12482586-Humans,
pubmed-meshheading:12482586-Immunosuppressive Agents,
pubmed-meshheading:12482586-Interleukin-2,
pubmed-meshheading:12482586-Peptides,
pubmed-meshheading:12482586-Protein Binding,
pubmed-meshheading:12482586-RNA Cap Analogs,
pubmed-meshheading:12482586-Sirolimus,
pubmed-meshheading:12482586-T-Lymphocytes,
pubmed-meshheading:12482586-Transfection
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pubmed:year |
2002
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pubmed:articleTitle |
Localisation and regulation of the eIF4E-binding protein 4E-BP3.
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pubmed:affiliation |
Division of Molecular Physiology, School of Life Sciences, University of Dundee, DD1 5EH, Dundee, UK. m.scheperkleijn@dundee.ac.uk
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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